ID   1.1.1.375
DE   L-2-hydroxycarboxylate dehydrogenase [NAD(P)(+)].
AN   lactate/malate dehydrogenase.
CA   (1) a (2S)-2-hydroxycarboxylate + NAD(+) = a 2-oxocarboxylate + H(+) +
CA   NADH.
CA   (2) a (2S)-2-hydroxycarboxylate + NADP(+) = a 2-oxocarboxylate + H(+) +
CA   NADPH.
CC   -!- The enzyme from the archaeon Methanocaldococcus jannaschii catalyzes
CC       the reversible oxidation of (2R)-3-sulfolactate and (S)-malate to
CC       3-sulfopyruvate and oxaloacetate, respectively (note that (2R)-3-
CC       sulfolactate has the same stereochemical configuration as (2S)-2-
CC       hydroxycarboxylates).
CC   -!- The enzyme can use both NADH and NADPH, although activity is higher
CC       with NADPH.
CC   -!- The oxidation of (2R)-3-sulfolactate was observed only in the
CC       presence of NADP(+).
CC   -!- The same organism also possesses an NAD(+)-specific enzyme with
CC       similar activity, cf. EC 1.1.1.337.
DR   Q60176, MDH_METJA  ;
//