ID 1.1.99.29 DE pyranose dehydrogenase (acceptor). AN PDH. AN pyranose dehydrogenase. AN pyranose-quinone oxidoreductase. AN quinone-dependent pyranose dehydrogenase. CA (1) pyranose + acceptor = pyranos-2-ulose + reduced acceptor. CA (2) pyranose + acceptor = pyranos-3-ulose + reduced acceptor. CA (3) pyranose + acceptor = pyranos-2,3-diulose + reduced acceptor. CA (4) a pyranoside + acceptor = a pyranosid-3-ulose + reduced acceptor. CA (5) a pyranoside + acceptor = a pyranosid-3,4-diulose + reduced acceptor. CC -!- A number of aldoses and ketoses in pyranose form, as well as CC glycosides, gluco-oligosaccharides, sucrose and lactose can act as a CC donor. CC -!- 1,4-Benzoquinone or ferricenium ion (ferrocene oxidized by removal of CC one electron) can serve as acceptor. CC -!- Unlike EC 1.1.3.10, this fungal enzyme does not interact with O2 and CC exhibits extremely broad substrate tolerance with variable CC regioselectivity (C-3, C-2 or C-3 + C-2 or C-3 + C-4) for CC (di)oxidation of different sugars. CC -!- D-glucose is exclusively or preferentially oxidized at C-3 (depending CC on the enzyme source), but can also be oxidized at C-2 + C-3. CC -!- The enzyme also acts on 1->4-alpha- and 1->4-beta-gluco- CC oligosaccharides, non-reducing gluco-oligosaccharides and CC L-arabinose, which are not substrates of EC 1.1.3.10. CC -!- Sugars are oxidized in their pyranose but not in their furanose form. DR Q3L1D1, PDH1_AGABI ; V5NDL4, PDH1_AGACM ; V5NC32, PDH1_AGAXA ; DR Q3L245, PDH1_LEUMG ; Q3L243, PDH2_LEUMG ; Q0R4L2, PDH3_LEUMG ; //