ID   1.13.12.5
DE   Renilla-type luciferase.
AN   luciferase.
AN   Renilla-luciferin 2-monooxygenase.
CA   coelenterazine h + O2 = CO2 + excited coelenteramide h monoanion + H(+) +
CA   hnu.
CC   -!- This enzyme has been studied from the soft coral Renilla reniformis.
CC   -!- Before the reaction occurs the substrate is sequestered by a
CC       coelenterazine-binding protein.
CC   -!- Elevation in the concentration of calcium ions releases the
CC       substrate, which then interacts with the luciferase.
CC   -!- Upon binding the substrate, the enzyme catalyzes an oxygenation,
CC       producing a very short-lived hydroperoxide that cyclizes into a
CC       dioxetanone structure, which collapses, releasing a CO2 molecule.
CC   -!- The spontaneous breakdown of the dioxetanone releases the energy
CC       (about 50 kcal/mole) that is necessary to generate the excited state
CC       of the coelenteramide product, which is the singlet form of the
CC       monoanion.
CC   -!- In vivo the product undergoes the process of nonradiative energy
CC       transfer to an accessory protein, a green fluorescent protein (GFP),
CC       which results in green bioluminescence.
CC   -!- In vitro, in the absence of GFP, the product emits blue light.
DR   P27652, LUCI_RENRE ;
//