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ENZYME entry: EC 1.14.13.142

Accepted Name
3-ketosteroid 9-alpha-monooxygenase.
Alternative Name(s)
3-ketosteroid 9-alpha-hydroxylase.
Reaction catalysed
Androsta-1,4-diene-3,17-dione + NADH + O(2) <=> 9-alpha-hydroxyandrosta-1,4-diene-3,17-dione + NAD(+) + H(2)O
Cofactor(s)
Fe cation; Iron-sulfur.
Comment(s)
  • The enzyme is involved in the cholesterol degradation pathway of several bacterial pathogens, such as Mycobacterium tuberculosis.
  • It is a two-component system consisting of a terminal oxygenase (KshA) and a ferredoxin reductase (KshB).
  • The oxygenase contains a Rieske-type iron-sulfur center and non-heme iron.
  • The reductase component is a flavoprotein containing an NAD-binding domain and a plant-type iron-sulfur cluster.
  • The product of the enzyme is unstable, and spontaneously converts to 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione.
Cross-references
BRENDA1.14.13.142
EC2PDB1.14.13.142
ExplorEnz1.14.13.142
PRIAM enzyme-specific profiles1.14.13.142
KEGG Ligand Database for Enzyme Nomenclature1.14.13.142
IUBMB Enzyme Nomenclature1.14.13.142
IntEnz1.14.13.142
MEDLINEFind literature relating to 1.14.13.142
MetaCyc1.14.13.142
UniProtKB/Swiss-Prot
F1CMX0, KSHA1_RHORH;  F1CMX8, KSHA3_RHORH;  B6V6V5, KSHA4_RHORH;  
F1CMY8, KSHA5_RHORH;  A0R4R3, KSHA_MYCS2;  P71875, KSHA_MYCTU;  
A0R525, KSHB_MYCS2;  P9WJ92, KSHB_MYCTO;  P9WJ93, KSHB_MYCTU;  
B6V6V6, KSHB_RHORH;  

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