ENZYME

ENZYME entry: EC 1.14.13.88

Accepted Name

Flavonoid 3',5'-hydroxylase.

Alternative Name(s)

F3',5'H.

F3'5'H.

Reaction catalysed

A flavanone + 2 NADPH + 2 O(2) <=> a 3',5'-dihydroxyflavanone + 2 NADP(+) + 2 H(2)O

Cofactor(s)

Heme-thiolate.

Comment(s)

The 3',5'-dihydroxyflavanone is formed via the 3'-hydroxyflavanone.
In Petunia hybrida the enzyme acts on naringenin, eriodictyol, dihydroquercetin (taxifolin) and dihydrokaempferol (aromadendrin).
The enzyme catalyzes the hydroxylation of 5,7,4'-trihydroxyflavanone (naringenin) at either the 3' position to form eriodictyol or at both the 3' and 5' positions to form 5,7,3',4',5'-pentahydroxyflavanone (dihydrotricetin).
The enzyme also catalyzes the hydroxylation of 3,5,7,3',4'-pentahydroxyflavanone (taxifolin) at the 5' position, forming ampelopsin.
NADH is not a good substitute for NADPH.

Cross-references

PROSITE

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature