ENZYME entry: EC 1.14.13.88

Accepted Name
Flavonoid 3',5'-hydroxylase.
Alternative Name(s)
F3',5'H.
F3'5'H.
Reaction catalysed
A flavanone + 2 NADPH + 2 O(2) <=> a 3',5'-dihydroxyflavanone + 2 NADP(+) + 2 H(2)O
Cofactor(s)
Heme-thiolate.
Comment(s)
  • The 3',5'-dihydroxyflavanone is formed via the 3'-hydroxyflavanone.
  • In Petunia hybrida the enzyme acts on naringenin, eriodictyol, dihydroquercetin (taxifolin) and dihydrokaempferol (aromadendrin).
  • The enzyme catalyzes the hydroxylation of 5,7,4'-trihydroxyflavanone (naringenin) at either the 3' position to form eriodictyol or at both the 3' and 5' positions to form 5,7,3',4',5'-pentahydroxyflavanone (dihydrotricetin).
  • The enzyme also catalyzes the hydroxylation of 3,5,7,3',4'-pentahydroxyflavanone (taxifolin) at the 5' position, forming ampelopsin.
  • NADH is not a good substitute for NADPH.
Cross-references
PROSITEPDOC00081
BRENDA1.14.13.88
EC2PDB1.14.13.88
ExplorEnz1.14.13.88
PRIAM enzyme-specific profiles1.14.13.88
KEGG Ligand Database for Enzyme Nomenclature1.14.13.88
IUBMB Enzyme Nomenclature1.14.13.88
IntEnz1.14.13.88
MEDLINEFind literature relating to 1.14.13.88
MetaCyc1.14.13.88
UniProtKB/Swiss-Prot
P48418, C75A1_PETHY;  P37120, C75A2_SOLME;  P48419, C75A3_PETHY;  
Q96581, C75A4_GENTR;  Q96418, C75A5_EUSER;  O04773, C75A6_CAMME;  
O04790, C75A7_EUSER;  

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