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ENZYME

ENZYME entry: EC 1.14.14.139

Accepted Name
5beta-cholestane-3alpha,7alpha-diol 12alpha-hydroxylase
Alternative Name(s)
5beta-cholestane-3alpha,7alpha-diol 12alpha-monooxygenase
7alpha-hydroxy-4-cholesten-3-one 12alpha-monooxygenase
7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase
chenodeoxycholate 12alpha monooxygenase
CYP8B1
sterol 12alpha-hydroxylase
Reaction catalysed
  • 5beta-cholestane-3alpha,7alpha-diol + O2 + reduced [NADPH--hemoprotein reductase] <=> 5beta-cholestane-3alpha,7alpha,12alpha-triol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase]
  • 7alpha-hydroxycholest-4-en-3-one + O2 + reduced [NADPH--hemoprotein reductase] <=> 7alpha,12alpha-dihydroxycholest-4-en-3-one + H(+) + H2O + oxidized [NADPH--hemoprotein reductase]
  • chenodeoxycholate + O2 + reduced [NADPH--hemoprotein reductase] <=> cholate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase]
Comment(s)
  • A cytochrome P450 (heme-thiolate) protein found in mammals.
  • This is the key enzyme in the biosynthesis of the bile acid cholate.
  • The enzyme can also hydroxylate 5beta-cholestane-3alpha,7alpha-diol at the 25 and 26 position, but to a lesser extent.
  • Formerly EC 1.14.13.96 and EC 1.14.18.8.
Cross-references
BRENDA1.14.14.139
EC2PDB1.14.14.139
ExplorEnz1.14.14.139
PRIAM enzyme-specific profiles1.14.14.139
KEGG Ligand Database for Enzyme Nomenclature1.14.14.139
IUBMB Enzyme Nomenclature1.14.14.139
IntEnz1.14.14.139
MEDLINEFind literature relating to 1.14.14.139
MetaCyc1.14.14.139
Rhea expert-curated reactions1.14.14.139
UniProtKB/Swiss-Prot
Q9UNU6, CP8B1_HUMANO88962, CP8B1_MOUSEQ7YRB2, CP8B1_PIG
O02766, CP8B1_RABIT

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