Expasy logo

ENZYME

ENZYME entry: EC 1.14.14.43

Accepted Name
(methylsulfanyl)alkanaldoxime N-monooxygenase
Alternative Name(s)
(methylthio)alkanaldoxime N-monooxygenase
CYP83A1
Reaction catalysed
an (E)-omega-(methylsulfanyl)-alkanal oxime + glutathione + O2 + reduced [NADPH--hemoprotein reductase] <=> an S-[(1E)-1-(hydroxyimino)-omega-(methylsulfanyl)alkyl]-L-glutathione + H(+) + 2 H2O + oxidized [NADPH--hemoprotein reductase]
Comment(s)
  • Involved in the biosynthesis of glucosinolates in plants.
  • Catalyzes an N-hydroxylation of the E isomer of n-(methylsulfanyl)aldoximes, forming an aci-nitro intermediate that reacts non-enzymically with glutathione to produce an N-alkyl- thiohydroximate adduct, the committed precursor of glucosinolates.
  • In the absence of a thiol compound, the enzyme is suicidal, probably due to interaction of the reactive aci-nitro intermediate with active site residues.
Cross-references
BRENDA1.14.14.43
EC2PDB1.14.14.43
ExplorEnz1.14.14.43
PRIAM enzyme-specific profiles1.14.14.43
KEGG Ligand Database for Enzyme Nomenclature1.14.14.43
IUBMB Enzyme Nomenclature1.14.14.43
IntEnz1.14.14.43
MEDLINEFind literature relating to 1.14.14.43
MetaCyc1.14.14.43
Rhea expert-curated reactions1.14.14.43
UniProtKB/Swiss-Prot
P48421, C83A1_ARATH

View entry in original ENZYME format
View entry in raw text format (no links)
All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.14.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.-.-.-