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ENZYME entry: EC 1.14.99.38

Accepted Name
Cholesterol 25-hydroxylase.
Alternative Name(s)
Cholesterol 25-monooxygenase.
Reaction catalysed
Cholesterol + AH(2) + O(2) <=> 25-hydroxycholesterol + A + H(2)O
Cofactor(s)
Fe cation.
Comment(s)
  • Unlike most other sterol hydroxylases, this enzyme is not a cytochrome P450.
  • Instead, it uses diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
  • The diiron cofactor can be either Fe-O-Fe or Fe-OH-Fe and is bound to the enzyme through interactions with clustered histidine or glutamate residues.
  • In cell cultures, this enzyme down-regulates cholesterol synthesis and the processing of sterol regulatory element binding proteins (SREBPs).
Cross-references
BRENDA1.14.99.38
EC2PDB1.14.99.38
ExplorEnz1.14.99.38
PRIAM enzyme-specific profiles1.14.99.38
KEGG Ligand Database for Enzyme Nomenclature1.14.99.38
IUBMB Enzyme Nomenclature1.14.99.38
IntEnz1.14.99.38
MEDLINEFind literature relating to 1.14.99.38
MetaCyc1.14.99.38
UniProtKB/Swiss-Prot
O95992, CH25H_HUMAN;  Q9Z0F5, CH25H_MOUSE;  Q4G1G8, CH25H_PIG;  
Q4QQV7, CH25H_RAT;  P11712, CP2C9_HUMAN;  

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