ID 1.17.3.4 DE juglone 3-hydroxylase. AN naphthoquinone hydroxylase. CA 2 juglone + O2 = 2 3,5-dihydroxy-1,4-naphthoquinone + 2 H(+). CC -!- Even though oxygen is consumed, molecular oxygen is not incorporated CC into the product. CC -!- Catalysis starts by incorporation of an oxygen atom from a water CC molecule into the substrate. CC -!- The naphthalene-1,2,4,8-tetrol intermediate is then oxidized by CC molecular oxygen, which is reduced to water. CC -!- Also acts on 1,4-naphthoquinone, naphthazarin and 2-chloro-1,4- CC naphthoquinone. CC -!- Formerly EC 1.14.99.27. //