ID   1.17.99.3
DE   3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA
DE   24-hydroxylase.
AN   3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate
AN   24-hydroxylase.
AN   3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA oxidase.
AN   THC-CoA oxidase.
AN   THCA-CoA oxidase.
AN   Trihydroxycoprostanoyl-CoA oxidase.
CA   (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oyl-CoA +
CA   H(2)O + acceptor = (24R,25R)-3-alpha,7-alpha,12-alpha,24-tetrahydroxy-5-
CA   beta-cholestan-26-oyl-CoA + reduced acceptor.
CC   -!- Requires ATP.
CC   -!- The reaction in mammals possibly involves dehydrogenation to give a
CC       24(25)-double bond followed by hydration.
CC   -!- However, in amphibians such as the Oriental fire-bellied toad
CC       (Bombina orientalis), it is probable that the product is formed via
CC       direct hydroxylation of the saturated side chain of (25R)-3-alpha,7-
CC       alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate and not via
CC       hydration of a 24(25) double bond.
CC   -!- In microsomes, the free acid is preferred to the coenzyme A ester,
CC       whereas in mitochondria, the coenzyme A ester is preferred to the
CC       free-acid form of the substrate.
DR   Q99424, ACOX2_HUMAN;  Q9QXD1, ACOX2_MOUSE;  O02767, ACOX2_RABIT;
DR   P97562, ACOX2_RAT  ;
//