ID   1.2.3.3
DE   Pyruvate oxidase.
AN   Phosphate-dependent pyruvate oxidase.
AN   Pyruvic oxidase.
CA   Pyruvate + phosphate + O(2) = acetyl phosphate + CO(2) + H(2)O(2).
CF   FAD; Thiamine diphosphate.
CC   -!- Two reducing equivalents are transferred from the resonant
CC       carbanion/enamine forms of 2-hydroxyethyl-thiamine-diphosphate to the
CC       adjacent flavin cofactor, yielding 2-acetyl-thiamine diphosphate
CC       (AcThDP) and reduced flavin.
CC   -!- FADH is reoxidized by O(2) to yield H(2)O(2) and FAD and AcThDP is
CC       cleaved phosphorolytically to acetyl phosphate and thiamine
CC       diphosphate.
PR   PROSITE; PDOC00166;
DR   P37063, POXB_LACPL ;  Q54970, POXB_STRPN ;
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