ID   1.3.99.8
DE   2-furoyl-CoA dehydrogenase.
AN   furoyl-CoA hydroxylase.
CA   2-furoyl-CoA + A + H2O = 5-hydroxy-2-furoyl-CoA + AH2 + H(+).
CC   -!- The oxygen atom of the -OH produced is derived from water, not O2.
CC   -!- The actual oxidative step is probably dehydrogenation of a hydrated
CC       form -CHOH-CH2- to -C(OH)=CH-, which tautomerizes non-enzymically to
CC       -CO-CH2-, giving (5-oxo-4,5-dihydro-2-furoyl)-CoA.
CC   -!- Methylene blue, nitro blue tetrazolium and a membrane fraction from
CC       Pseudomonas putida can act as acceptors.
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