ENZYME

ENZYME entry: EC 1.5.1.28

Accepted Name

Opine dehydrogenase.

Reaction catalysed

(2S)-2-((1-(R)-carboxyethyl)amino)pentanoate + NAD(+) + H(2)O <=> L-2-aminopentanoic acid + pyruvate + NADH

Comment(s)

In the forward direction, the enzyme from Arthrobacter sp. acts also on secondary amine dicarboxylates such as N-(1-carboxyethyl)methionine and N-(1-carboxyethyl)phenylalanine.
Dehydrogenation forms an imine, which dissociates to the amino acid and pyruvate.
In the reverse direction, the enzyme acts also on neutral amino acids as an amino donor.
They include L-amino acids such as 2-aminopentanoic acid, 2-aminobutyric acid, 2-aminohexanoic acid, 3-chloroalanine, O-acetylserine, methionine, isoleucine, valine, phenylalanine, leucine and alanine.
The amino acceptors include 2-oxoacids such as pyruvate, oxaloacetate, glyoxylate and 2-oxobutyrate.

Cross-references

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature