ID   2.1.1.202
DE   multisite-specific tRNA:(cytosine-C(5))-methyltransferase.
CA   (1) cytidine(34) in tRNA precursor + S-adenosyl-L-methionine =
CA   5-methylcytidine(34) in tRNA precursor + H(+) + S-adenosyl-L-
CA   homocysteine.
CA   (2) cytidine(40) in tRNA precursor + S-adenosyl-L-methionine =
CA   5-methylcytidine(40) in tRNA precursor + H(+) + S-adenosyl-L-
CA   homocysteine.
CA   (3) cytidine(48) in tRNA + S-adenosyl-L-methionine = 5-methylcytidine(48)
CA   in tRNA + H(+) + S-adenosyl-L-homocysteine.
CA   (4) cytidine(49) in tRNA + S-adenosyl-L-methionine = 5-methylcytidine(49)
CA   in tRNA + H(+) + S-adenosyl-L-homocysteine.
CC   -!- The enzyme from Saccharomyces cerevisiae is responsible for complete
CC       5-methylcytosine methylations of yeast tRNA.
CC   -!- The incidence of modification depends on the cytosine position in
CC       tRNA.
CC   -!- At positions 34 and 40, 5-methylcytosine is found only in two yeast
CC       tRNAs (tRNA(Leu)(CUA) and tRNA(Phe)(GAA), respectively), whereas most
CC       other elongator yeast tRNAs bear either 5-methylcytosine(48) or
CC       5-methylcytosine(49), but never both in the same tRNA molecule.
CC   -!- The formation of 5-methylcytosine(34) and 5-methylcytosine(40) is a
CC       strictly intron-dependent process, whereas the formation of
CC       5-methylcytosine(48) and 5-methylcytosine(49) is an intron-
CC       independent process.
CC   -!- Formerly EC 2.1.1.29.
DR   P38205, NCL1_YEAST ;
//