ENZYME entry: EC 2.1.1.224

Accepted Name
23S rRNA (adenine(2503)-C(8))-methyltransferase.
Reaction catalysed
2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin <=> S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine(2503) in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
Cofactor(s)
Iron-sulfur.
Comment(s)
  • This enzyme is a member of the 'AdoMet radical' (radical SAM) family.
  • S-adenosyl-L-methionine acts as both a radical generator and as the source of the appended methyl group.
  • Cfr is an plasmid-acquired methyltransferase that protects cells from the action of antibiotics.
  • The enzyme methylates adenosine at position 2503 of 23S rRNA by a radical mechanism, transferring a CH(2) group from S-adenosyl-L- methionine while retaining the hydrogen at the C-8 position of the adenine.
  • Cfr first transfers an CH(2) group to a conserved cysteine (Cys(338) in Staphylococcus aureus), the generated radical from a second S-adenosyl-L-methionine then attacks the methyl group, exctracting a hydrogen.
  • The formed radical forms a covalent intermediate with the adenine group of the tRNA.
  • The enzyme will also methylate 2-methyladenine produced by the action of EC 2.1.1.192.
Cross-references
BRENDA2.1.1.224
EC2PDB2.1.1.224
ExplorEnz2.1.1.224
PRIAM enzyme-specific profiles2.1.1.224
KEGG Ligand Database for Enzyme Nomenclature2.1.1.224
IUBMB Enzyme Nomenclature2.1.1.224
IntEnz2.1.1.224
MEDLINEFind literature relating to 2.1.1.224
MetaCyc2.1.1.224
UniProtKB/Swiss-Prot
Q5WJ42, CFR_BACSK;  A7Z1T2, CFR_BACVZ;  A7FX96, CFR_CLOB1;  
A5I5U3, CFR_CLOBH;  B1IL14, CFR_CLOBK;  A7GH77, CFR_CLOBL;  
B1KZ37, CFR_CLOBM;  A9KK15, CFR_CLOPH;  A5HBL2, CFR_STAAU;  
Q9FBG4, CFR_STASC;  A2AXI2, CFR_STAWA;  

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