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ENZYME entry: EC

Accepted Name
23S rRNA (adenine(2503)-C(8))-methyltransferase.
Reaction catalysed
2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin <=> S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine(2503) in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
  • This enzyme is a member of the 'AdoMet radical' (radical SAM) family.
  • S-adenosyl-L-methionine acts as both a radical generator and as the source of the appended methyl group.
  • Cfr is an plasmid-acquired methyltransferase that protects cells from the action of antibiotics.
  • The enzyme methylates adenosine at position 2503 of 23S rRNA by a radical mechanism, transferring a CH(2) group from S-adenosyl-L- methionine while retaining the hydrogen at the C-8 position of the adenine.
  • Cfr first transfers an CH(2) group to a conserved cysteine (Cys(338) in Staphylococcus aureus), the generated radical from a second S-adenosyl-L-methionine then attacks the methyl group, exctracting a hydrogen.
  • The formed radical forms a covalent intermediate with the adenine group of the tRNA.
  • The enzyme will also methylate 2-methyladenine produced by the action of EC
PRIAM enzyme-specific profiles2.1.1.224
KEGG Ligand Database for Enzyme Nomenclature2.1.1.224
IUBMB Enzyme Nomenclature2.1.1.224
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