ID 2.1.1.254 DE erythromycin 3''-O-methyltransferase. CA (1) erythromycin C + S-adenosyl-L-methionine = erythromycin A + H(+) + CA S-adenosyl-L-homocysteine. CA (2) erythromycin D + S-adenosyl-L-methionine = erythromycin B + H(+) + CA S-adenosyl-L-homocysteine. CC -!- The enzyme methylates the 3 position of the mycarosyl moiety of CC erythromycin C, forming the most active form of the antibiotic, CC erythromycin A. CC -!- It can also methylate the precursor erythromycin D, forming CC erythromycin B, which is then converted to erythromycin A by CC EC 1.14.13.154. DR A4F7P5, ERYG_SACEN ; //