ID   2.3.1.111
DE   mycocerosate synthase.
CA   (1) 3 (R)-methylmalonyl-CoA + 9 H(+) + long-chain fatty acyl-
CA   [mycocerosate synthase] + 6 NADPH = 3 CO2 + 3 CoA + 3 H2O + 6 NADP(+) +
CA   trimethylated-mycocerosoyl-[mycocerosate synthase].
CA   (2) 4 (R)-methylmalonyl-CoA + 12 H(+) + long-chain fatty acyl-
CA   [mycocerosate synthase] + 8 NADPH = 4 CO2 + 4 CoA + 4 H2O + 8 NADP(+) +
CA   tetramethylated-mycocerosoyl-[mycocerosate synthase].
CC   -!- The enzyme, characterized from mycobacteria, is loaded with a long-
CC       chain acyl moiety by EC 6.2.1.49 and elongates it by incorporation of
CC       three or four methylmalonyl (but not malonyl) residues, to form tri-
CC       or tetramethyl-branched fatty-acids, respectively, such as 2,4,6,8-
CC       tetramethyloctacosanoate (C32-mycocerosate).
CC   -!- Since the enzyme lacks a thioesterase domain, the product remains
CC       bound and requires additional enzyme(s) for removal.
CC   -!- Even though the enzyme can accept C6 to C20 substrates in vitro,
CC       it prefers to act on C14-C20 substrates in vivo.
DR   Q02251, MCAS_MYCBO ;
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