ID   2.4.1.229
DE   [Skp1-protein]-hydroxyproline N-acetylglucosaminyltransferase.
AN   Skp1-HyPro GlcNAc-transferase.
AN   UDP-GlcNAc:hydroxyproline polypeptide GlcNAc-transferase.
AN   UDP-GlcNAc:Skp1-hydroxyproline GlcNAc-transferase.
AN   UDP-N-acetylglucosamine (GlcNAc):hydroxyproline polypeptide GlcNAc-
AN   transferase.
CA   UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline = UDP + [Skp1-
CA   protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline.
CC   -!- Requires dithiothreitol and a divalent cation for activity.
CC   -!- This enzyme commences the building up of a pentasaccharide (Gal-
CC       alpha-1-6Gal-alpha-1-L-Fuc-alpha-1-2Gal-beta-1-3GlcNAc) on Hyp-143 of
CC       the Dictyostelium protein Skp1, which is required for the
CC       ubiquitination of cell-cycle regulatory proteins and transcription
CC       factors.
CC   -!- The fucose residue is probably in the alpha configuration.
CC   -!- The specificity of the enzyme for Skp1-Hyp-143 and its high affinity
CC       for this substrate suggests that it is the GlcNAc-transferase that
CC       modifies Skp1 in vivo.
DR   Q8T1C6, GNT1_DICDI ;
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