ENZYME

ENZYME entry: EC 2.4.1.266

Accepted Name

Glucosyl-3-phosphoglycerate synthase.

Alternative Name(s)

Glucosylphosphoglycerate synthase.

GPG synthase.

Reaction catalysed

NDP-glucose + 3-phospho-D-glycerate <=> NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate

Comment(s)

The enzyme is involved in biosynthesis of 2-O-(alpha-D- glucopyranosyl)-D-glycerate via the two-step pathway in which glucosyl-3-phosphoglycerate synthase catalyzes the conversion of GDP- glucose and 3-phospho-D-glycerate into 2-O-(alpha-D-glucopyranosyl)- 3-phospho-D-glycerate, which is then converted to 2-O-(alpha-D- glucopyranosyl)-D-glycerate by EC 3.1.3.85.
The activity is dependent on divalent cations (Mn(2+), Co(2+), or Mg(2+)).
The enzyme from Persephonella marina shows moderate flexibility on the sugar donor concerning the nucleotide moiety (UDP-glucose, ADP- glucose, GDP-glucose) but is strictly specific for glucose.
The enzyme is also strictly specific for 3-phospho-D-glycerate as acceptor.
The enzyme from Methanococcoides burtonii is strictly specific for GDP-glucose and 3-phospho-D-glycerate.
This enzyme catalyzes the first glucosylation step in methylglucose lipopolysaccharide biosynthesis in mycobacteria.

Cross-references

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature