ENZYME entry: EC 2.4.99.18

Accepted Name
Dolichyl-diphosphooligosaccharide--protein glycotransferase.
Reaction catalysed
Dolichyl diphosphooligosaccharide + [protein]-L-asparagine <=> dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
Comment(s)
  • Occurs in eukaryotes that form a glycoprotein by the transfer of a glucosyl-mannosyl-glucosamine polysaccharide to the side-chain of an L-asparagine residue in the sequence -Asn-Xaa-Ser- or -Asn-Xaa-Thr- (Xaa not Pro) in nascent polypeptide chains.
  • The basic oligosaccharide is the tetradecasaccharide Glc(3)Man(9)GlcNAc(2).
  • However, smaller oligosaccharides derived from it and oligosaccharides with additional monosaccharide units attached may be involved.
  • Man(3)GlcNAc(2) seems to be common for all of the oligosaccharides involved with the terminal N-acetylglucosamine linked to the protein L-asparagine.
  • Occurs on the cytosolic face of the endoplasmic reticulum.
  • The dolichol involved normally has 14-21 isoprenoid units with two trans double-bonds at the omega end, and the rest of the double-bonds in cis form.
  • Formerly EC 2.4.1.119.
Cross-references
PROSITEPDOC00172
BRENDA2.4.99.18
EC2PDB2.4.99.18
ExplorEnz2.4.99.18
PRIAM enzyme-specific profiles2.4.99.18
KEGG Ligand Database for Enzyme Nomenclature2.4.99.18
IUBMB Enzyme Nomenclature2.4.99.18
IntEnz2.4.99.18
MEDLINEFind literature relating to 2.4.99.18
MetaCyc2.4.99.18
UniProtKB/Swiss-Prot
Q39080, DAD1_ARATH;  Q8I7Z2, DAD1_ARAVE;  Q9M3T9, DAD1_BETPN;  
P52872, DAD1_CAEEL;  Q9ZWQ7, DAD1_CITUN;  Q54FB6, DAD1_DICDI;  
Q9VLM5, DAD1_DROME;  Q9SME9, DAD1_HORVU;  O81214, DAD1_MAIZE;  
O24060, DAD1_MALDO;  A2XSY1, DAD1_ORYSI;  Q0JDK9, DAD1_ORYSJ;  
Q9ZRA3, DAD1_PEA;  O65085, DAD1_PICMA;  Q9SMC4, DAD1_SOLLC;  
P46967, DAD1_XENLA;  O22622, DAD2_ARATH;  Q9SME8, DAD2_HORVU;  
P12244, GSBP_CHICK;  Q9SFX3, OST1A_ARATH;  Q0DJC5, OST1A_ORYSJ;  
Q9ZUA0, OST1B_ARATH;  B9FDT1, OST1B_ORYSJ;  Q54C27, OST1_DICDI;  
Q10176, OST1_SCHPO;  P41543, OST1_YEAST;  O14238, OST2_SCHPO;  
P46964, OST2_YEAST;  Q8TFH3, OST3_SCHPO;  P48439, OST3_YEAST;  
Q944K2, OST48_ARATH;  P45971, OST48_CAEEL;  Q6NYS8, OST48_DANRE;  
Q54E62, OST48_DICDI;  Q24319, OST48_DROME;  Q6ZLK0, OST48_ORYSJ;  
Q6GNR9, OST48_XENLA;  B1H3C9, OST48_XENTR;  Q9P838, OST4_CANAX;  
Q96VG2, OST4_SCHPO;  Q99380, OST4_YEAST;  G2TRU0, OST5_SCHPO;  
Q92316, OST5_YEAST;  Q03723, OST6_YEAST;  O59866, OSTB_SCHPO;  
P33767, OSTB_YEAST;  Q02795, OSTD_YEAST;  Q9GZH4, RPN1_CAEEL;  
P80896, RPN1_CHICK;  Q93Z16, RPN2_ARATH;  P91390, RPN2_CAEEL;  
Q54HG9, RPN2_DICDI;  Q5N7W3, RPN2_ORYSJ;  Q93ZY3, STT3A_ARATH;  
Q2KJI2, STT3A_BOVIN;  F1PJP5, STT3A_CANLF;  P46977, STT3A_HUMAN;  
P46978, STT3A_MOUSE;  Q6F2Z1, STT3A_ORYSJ;  Q5RCE2, STT3A_PONAB;  
Q9FX21, STT3B_ARATH;  E2RG47, STT3B_CANLF;  Q8TCJ2, STT3B_HUMAN;  
Q3TDQ1, STT3B_MOUSE;  Q7XQ88, STT3B_ORYSJ;  P46975, STT3_CAEEL;  
Q54NM9, STT3_DICDI;  O94335, STT3_SCHPO;  P39007, STT3_YEAST;  

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