ENZYME entry: EC 2.5.1.77

Accepted Name
7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase.
Reaction catalysed
5-amino-6-(D-ribitylamino)uracil + 3-(4-hydroxyphenyl)pyruvate + 2 S-adenosyl-L-methionine + H(2)O <=> 7,8-didemethyl-8-hydroxy-5-deazariboflavin + 2 L-methionine + 2 5'-deoxyadenosine + oxalate + NH(3)
Cofactor(s)
Iron-sulfur.
Comment(s)
  • Binds a 4Fe-4S cluster.
  • The cluster is coordinated by 3 cysteines and an exchangeable SAM molecule.
  • The first stage of catalysis is reduction of the 2 AdoMet to produce 2 methionine and 2 5'-deoxyadenosin-5-yl radicals that extract a hydrogen from each of the substrates permitting the condensation of the two.
  • The overall reaction catalyzed is the transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino-6- ribitylaminopyrimidine-2,4(1H,3H)-dione to form 7,8-didemethyl-8- hydroxy-5-deazariboflavin (FO).
  • 7,8-didemethyl-8-hydroxy-5-deazariboflavin is the chromophore of the hydride carrier coenzyme F420.
Cross-references
BRENDA2.5.1.77
EC2PDB2.5.1.77
ExplorEnz2.5.1.77
PRIAM enzyme-specific profiles2.5.1.77
KEGG Ligand Database for Enzyme Nomenclature2.5.1.77
IUBMB Enzyme Nomenclature2.5.1.77
IntEnz2.5.1.77
MEDLINEFind literature relating to 2.5.1.77
MetaCyc2.5.1.77
UniProtKB/Swiss-Prot
Q3MCR5, COFG_ANAVT;  O29461, COFG_ARCFU;  Q7NIT2, COFG_GLOVI;  
Q5UXN0, COFG_HALMA;  B0R6P8, COFG_HALS3;  Q9HNU9, COFG_HALSA;  
A6UVH0, COFG_META3;  Q8TQP9, COFG_METAC;  A7IAF4, COFG_METB6;  
Q466A4, COFG_METBF;  Q12WF6, COFG_METBU;  Q57888, COFG_METJA;  
Q8TWY3, COFG_METKA;  A4FXR1, COFG_METM5;  A9AB67, COFG_METM6;  
A6VFG8, COFG_METM7;  Q8PU55, COFG_METMA;  A3CWL4, COFG_METMJ;  
Q6LYV9, COFG_METMP;  B8GGS3, COFG_METPE;  A5UM12, COFG_METS3;  
O27266, COFG_METTH;  A0B5P5, COFG_METTP;  A6UN87, COFG_METVS;  
Q8YR77, COFG_NOSS1;  Q31PU7, COFG_SYNE7;  Q5N4D1, COFG_SYNP6;  
P73191, COFG_SYNY3;  Q8DIR6, COFG_THEEB;  Q11A09, COFG_TRIEI;  
Q8TQQ0, COFH1_METAC;  Q466A3, COFH1_METBF;  Q8PU54, COFH1_METMA;  
Q6M161, COFH1_METMP;  Q8TQQ1, COFH2_METAC;  Q466A2, COFH2_METBF;  
Q8PU53, COFH2_METMA;  Q6M160, COFH2_METMP;  Q3MF37, COFH_ANAVT;  
O29460, COFH_ARCFU;  B8HP19, COFH_CYAP4;  Q7NMI9, COFH_GLOVI;  
Q5UXN1, COFH_HALMA;  Q9HNU8, COFH_HALSA;  A6UV11, COFH_META3;  
Q12WF5, COFH_METBU;  Q2FSC7, COFH_METHJ;  Q58826, COFH_METJA;  
Q8TWY4, COFH_METKA;  A4G0D4, COFH_METM5;  A9A8N5, COFH_METM6;  
A6VI43, COFH_METM7;  A3CRI3, COFH_METMJ;  B8GI94, COFH_METPE;  
O26911, COFH_METTH;  A6UR49, COFH_METVS;  B0JTF9, COFH_MICAN;  
B2IW30, COFH_NOSP7;  Q8YPR3, COFH_NOSS1;  Q31QF1, COFH_SYNE7;  
Q5N3T7, COFH_SYNP6;  P72811, COFH_SYNY3;  Q8DII8, COFH_THEEB;  
Q113H3, COFH_TRIEI;  Q0W6V4, COFH_UNCMA;  Q7U0G9, FBIC_MYCBO;  
Q9CBX6, FBIC_MYCLE;  Q73WP9, FBIC_MYCPA;  O50429, FBIC_MYCTU;  
Q5YQD7, FBIC_NOCFA;  Q82GV2, FBIC_STRAW;  Q9KZZ7, FBIC_STRCO;  

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