ID   2.7.1.160
DE   2'-phosphotransferase.
AN   Tpt1p.
AN   yeast 2'-phosphotransferase.
CA   2'-phospho-[ligated tRNA] + NAD(+) = ADP-alpha-D-ribose 1'',2''-cyclic
CA   phosphate + mature tRNA + nicotinamide.
CC   -!- Catalyzes the final step of tRNA splicing in the yeast Saccharomyces
CC       cerevisiae.
CC   -!- The reaction takes place in two steps: in the first step,
CC       the 2'-phosphate on the RNA substrate is ADP-ribosylated, causing the
CC       relase of nicotinamide and the formation of the reaction
CC       intermediate, ADP-ribosylated tRNA.
CC   -!- In the second step, dephosphorylated (mature) tRNA is formed along
CC       with ADP ribose 1'',2''-cyclic phosphate.
CC   -!- Highly specific for oligonucleotide substrates bearing an internal
CC       2'-phosphate.
CC   -!- Oligonucleotides with only a terminal 5'- or 3'-phosphate are not
CC       substrates.
DR   O14045, TPT1_SCHPO ;  Q12272, TPT1_YEAST ;  Q3ZBM7, TRPT1_BOVIN;
DR   Q5EAR5, TRPT1_DANRE;  Q86TN4, TRPT1_HUMAN;  Q8K3A2, TRPT1_MOUSE;
//