ENZYME entry: EC 2.7.7.63

Accepted Name
Lipoate--protein ligase.
Alternative Name(s)
Lipoate protein ligase.
Lipoate-protein ligase A.
LPL.
Reaction catalysed
  • ATP + lipoate <=> diphosphate + lipoyl-AMP
  • Lipoyl-AMP + protein <=> protein N(6)-(lipoyl)lysine + AMP
Cofactor(s)
Mg(2+).
Comment(s)
  • Both 6S- and 6R-lipoates can act as substrates but there is a preference for the naturally occurring R-form.
  • Selenolipoate, i.e. 5-(1,2-diselenolan-3-yl)pentanoic acid, and 6-sulfanyloctanoate can also act as substrates, but more slowly.
  • Responsible for lipoylation in the presence of exogenous lipoic acid.
  • Lipoylation is essential for the function of several key enzymes involved in oxidative metabolism, including pyruvate dehydrogenase (E(2) domain), 2-oxoglutarate dehydrogenase (E(2) domain), the branched-chain 2-oxoacid dehydrogenases and the glycine cleavage system (H protein).
  • Attaches lipoic acid to the lipoyl domains of these proteins.
  • It is likely that an alternative pathway, involving EC 2.3.1.181 and EC 2.8.1.8 is the normal route for lipoylation.
Cross-references
BRENDA2.7.7.63
EC2PDB2.7.7.63
ExplorEnz2.7.7.63
PRIAM enzyme-specific profiles2.7.7.63
KEGG Ligand Database for Enzyme Nomenclature2.7.7.63
IUBMB Enzyme Nomenclature2.7.7.63
IntEnz2.7.7.63
MEDLINEFind literature relating to 2.7.7.63
MetaCyc2.7.7.63
UniProtKB/Swiss-Prot
Q9HKT2, LPLAC_THEAC;  Q9HKT1, LPLAN_THEAC;  A0KMH0, LPLA_AERHH;  
Q9Y9E6, LPLA_AERPE;  A4SQ09, LPLA_AERS4;  P60809, LPLA_BDEBA;  
Q7VR65, LPLA_BLOFL;  Q492U9, LPLA_BLOPB;  A8ALX2, LPLA_CITK8;  
A7MIG6, LPLA_CROS8;  A7ZVS8, LPLA_ECO24;  B7UR14, LPLA_ECO27;  
B7MNJ3, LPLA_ECO45;  B7LEN2, LPLA_ECO55;  P60776, LPLA_ECO57;  
B7NW66, LPLA_ECO7I;  B7MTD2, LPLA_ECO81;  B7LXU8, LPLA_ECO8A;  
C4ZT68, LPLA_ECOBW;  B1XFJ6, LPLA_ECODH;  A8A8B4, LPLA_ECOHS;  
A1AJV3, LPLA_ECOK1;  Q0T8S7, LPLA_ECOL5;  Q8FA49, LPLA_ECOL6;  
B1IS33, LPLA_ECOLC;  P32099, LPLA_ECOLI;  B7NH54, LPLA_ECOLU;  
B6I6N3, LPLA_ECOSE;  B1LEJ1, LPLA_ECOSM;  Q1R257, LPLA_ECOUT;  
A4W6A2, LPLA_ENT38;  B7LNS6, LPLA_ESCF3;  B5Y273, LPLA_KLEP3;  
A6TI00, LPLA_KLEP7;  P47512, LPLA_MYCGE;  P75394, LPLA_MYCPN;  
C6DKP5, LPLA_PECCP;  Q1QEC2, LPLA_PSYCK;  B5F534, LPLA_SALA4;  
A9MRA3, LPLA_SALAR;  Q57G36, LPLA_SALCH;  B5FTD0, LPLA_SALDC;  
B5R2K1, LPLA_SALEP;  B5R9V4, LPLA_SALG2;  B4TH09, LPLA_SALHS;  
B4T4I0, LPLA_SALNS;  Q5PK29, LPLA_SALPA;  A9N7E6, LPLA_SALPB;  
C0Q7M8, LPLA_SALPC;  B5BAK2, LPLA_SALPK;  B4TU46, LPLA_SALSV;  
Q8Z0U0, LPLA_SALTI;  Q8ZJV1, LPLA_SALTY;  O13629, LPLA_SCHPO;  
A8GDS3, LPLA_SERP5;  B2TZR8, LPLA_SHIB3;  Q31SV2, LPLA_SHIBS;  
Q327K9, LPLA_SHIDS;  Q3YU08, LPLA_SHISS;  A5F8E4, LPLA_VIBC3;  
Q9KS71, LPLA_VIBCH;  C3LM83, LPLA_VIBCM;  B3LQ66, LPLA_YEAS1;  
B5VLD2, LPLA_YEAS6;  A6ZPT1, LPLA_YEAS7;  P47051, LPLA_YEAST;  
A1JPK8, LPLA_YERE8;  A7FHI0, LPLA_YERP3;  Q1C748, LPLA_YERPA;  
B2K5K7, LPLA_YERPB;  Q8ZDY2, LPLA_YERPE;  A9R087, LPLA_YERPG;  
Q1CII3, LPLA_YERPN;  A4TIN0, LPLA_YERPP;  Q66A10, LPLA_YERPS;  
B1JJ36, LPLA_YERPY;  O07608, LPLJ_BACSU;  

View entry in original ENZYME format
View entry in raw text format (no links)

All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.7.7.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.7.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.-.-.-