ID 2.8.4.5 DE tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase. AN methylthio-threonylcarbamoyl-adenosine transferase B. CA [sulfur carrier]-SH + AH2 + N(6)-L-threonylcarbamoyladenosine(37) in tRNA CA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L- CA threonylcarbamoyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur CA carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine. CC -!- The enzyme, which is a member of the S-adenosyl-L-methionine- CC dependent radical (radical AdoMet) enzymes superfamily, binds two CC [4Fe-4S] clusters as well as the transferred sulfur. CC -!- The sulfur donor is believed to be one of the [4Fe-4S] clusters, CC which is sacrificed in the process, so that in vitro the reaction is CC a single turnover. CC -!- The identity of the electron donor is not known. DR O29021, AMTAB_ARCFU; Q58277, AMTAB_METJA; O26914, AMTAB_METTH; DR Q9UXX9, AMTAB_PYRAB; O59545, AMTAB_PYRHO; Q8MXQ7, CDKAL_CAEEL; DR Q6PG34, CDKAL_DANRE; Q7K4W1, CDKAL_DROME; Q291H5, CDKAL_DROPS; DR Q5VV42, CDKAL_HUMAN; Q91WE6, CDKAL_MOUSE; Q6NS26, CDKAL_XENLA; DR Q6P4Y0, CDKAL_XENTR; O66772, MTAB_AQUAE ; P54462, MTAB_BACSU ; DR Q9Z874, MTAB_CHLPN ; Q49573, MTAB_MALIO ; Q9ZDB6, MTAB_RICPR ; DR Q9WZT7, MTAB_THEMA ; O83293, MTAB_TREPA ; //