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ENZYME

ENZYME entry: EC 3.2.1.178

Accepted Name
beta-porphyranase
Alternative Name(s)
endo-beta-porphyranase
porphyranase
Reaction catalysed
Hydrolysis of beta-D-galactopyranose-(1->4)-alpha-L-galactopyranose-6-sulfate linkages in porphyran
Comment(s)
  • The backbone of porphyran consists largely (approximately 70%) of (1->3)-linked beta-D-galactopyranose followed by (1->4)-linked alpha- L-galactopyranose-6-sulfate [the other 30% are mostly agarobiose repeating units of (1->3)-linked beta-D-galactopyranose followed by (1->4)-linked 3,6-anhydro-alpha-L-galactopyranose].
  • This enzyme cleaves the (1->4) linkages between beta-D- galactopyranose and alpha-L-galactopyranose-6-sulfate, forming mostly the disaccharide alpha-L-galactopyranose-6-sulfate-(1->3)-beta-D- galactose, although some longer oligosaccharides of even number of residues are also observed.
  • Since the enzyme is inactive on the non-sulfated agarose portion of the porphyran backbone, some agarose fragments are also included in the products.
  • Methylation of the D-galactose prevents the enzyme from Zobellia galactanivorans, but not that from Wenyingzhuangia fucanilytica, from binding at position -1.
Cross-references
BRENDA3.2.1.178
EC2PDB3.2.1.178
ExplorEnz3.2.1.178
PRIAM enzyme-specific profiles3.2.1.178
KEGG Ligand Database for Enzyme Nomenclature3.2.1.178
IUBMB Enzyme Nomenclature3.2.1.178
IntEnz3.2.1.178
MEDLINEFind literature relating to 3.2.1.178
MetaCyc3.2.1.178
Rhea expert-curated reactions3.2.1.178
UniProtKB/Swiss-Prot
B5CY96, PORA_PHOPMD7GXG0, PORA_ZOBGAB5CY92, PORB_PHOPM
D7GXF9, PORB_ZOBGAD7GXG1, PORC_ZOBGAD7GXG2, PORD_ZOBGA
D7GXG3, PORE_ZOBGA

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