ID 3.2.1.218 DE alpha-3'-ketoglucosidase. AN 3'-keto-alpha-D-gluco-disaccharide hydrolase. AN 3-keto-glucoside hydrolase. CA 3'-dehydrosucrose + H2O = 3-dehydro-D-glucopyranose + beta-D- CA fructofuranose. CC -!- The enzyme, originally characterized from the bacterium Agrobacterium CC tumefaciens, is specific for disaccharides that contain a 3-dehydro- CC alpha-D-glucose at the non-reducing end such as 3'-dehydrosucrose and CC 3'-dehydro-alpha,alpha-trehalose. CC -!- It has no activity with disaccharides in which the glucose is in beta CC conformation, and greatly reduced activity with disaccharides with an CC unmodified 3' position. //