ENZYME

Home | Contact

ENZYME entry: EC 3.2.1.97

Accepted Name

Endo-alpha-N-acetylgalactosaminidase.

Alternative Name(s)

D-galactosyl-3-(N-acetyl-alpha-D-galactosaminyl)-L-serine mucinaminohydrolase.

D-galactosyl-N-acetyl-alpha-D-galactosamine D-galactosyl-N-acetyl- galactosaminohydrolase.

Endo-alpha-acetylgalactosaminidase.

Endo-alpha-GalNAc-ase.

Endo-alpha-N-acetyl-D-galactosaminidase.

Glycopeptide alpha-N-acetylgalactosaminidase.

Mucinaminylserine mucinaminidase.

Reaction catalysed

3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H(2)O <=> 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]

Comment(s)

The enzyme catalyzes the liberation of Gal-(1->3)-beta-GalNAc alpha- linked to serine or threonine residues of mucin-type glycoproteins.
EngBF from Bifidobacterium longum specifically acts on core 1-type O-glycan to release the disaccharide Gal-(1->3)-beta-GalNAc.
The enzymes from Clostridium perfringens, Enterococcus faecalis, Propionibacterium acnes and Alcaligenes faecalis show broader specificity (e.g. release of the core 2 trisaccharide Gal-(1->3)- beta-(GlcNAc-(1->6)-beta)-GalNAc or the core 3 disaccharide GlcNAc- (1->3)-beta-GalNAc).
The enzyme may play an important role in the degradation and utilization of mucins having core 1 O-glycan.
Formerly EC 3.2.1.110.

Cross-references

BRENDA

3.2.1.97

EC2PDB

3.2.1.97

ExplorEnz

3.2.1.97

PRIAM enzyme-specific profiles

3.2.1.97

KEGG Ligand Database for Enzyme Nomenclature

3.2.1.97

IUBMB Enzyme Nomenclature