ENZYME

ENZYME entry: EC 3.4.13.22

Accepted Name

D-Ala-D-Ala dipeptidase.

Alternative Name(s)

D-alanyl-D-alanine dipeptidase.

Reaction catalysed

D-Ala-D-Ala + H(2)O <=> 2 D-Ala

Cofactor(s)

Zinc.

Comment(s)

Protects Enterococcus faecium from the antibiotic vancomycin, which can bind to the -D-Ala-D-Ala sequence at the C-terminus of the peptidoglycan pentapeptide.
Reduces the availability of the free dipeptide D-Ala-D-Ala, which is the precursor for this pentapeptide sequence, allowing D-Ala-(R)- lactate (for which vancomycin has much less affinity) to be added to the cell wall instead.
The enzyme is stereospecific, as L-Ala-L-Ala, D-Ala-L-Ala and L-Ala- D-Ala are not substrates.
Belongs to peptidase family M15.

Cross-references

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature