ENZYME entry: EC 3.4.22.44

Accepted Name
Nuclear-inclusion-a endopeptidase.
Alternative Name(s)
Potyvirus NIa protease.
Reaction catalysed
Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved
Comment(s)
  • The potyviruses cause diseases in plants, and inclusion bodies appear in the host cell nuclei, protein a of these being the endopeptidase.
  • The enzyme finds practical use when encoded in vectors for the artificial expression of recombinant fusion proteins, since it can confer on them the capacity for autolytic cleavage.
  • It is also reported that transgenic plants expressing the enzyme are resistant to viral infection.
  • Belongs to peptidase family C4.
Cross-references
BRENDA3.4.22.44
EC2PDB3.4.22.44
ExplorEnz3.4.22.44
PRIAM enzyme-specific profiles3.4.22.44
KEGG Ligand Database for Enzyme Nomenclature3.4.22.44
IUBMB Enzyme Nomenclature3.4.22.44
IntEnz3.4.22.44
MEDLINEFind literature relating to 3.4.22.44
MetaCyc3.4.22.44
UniProtKB/Swiss-Prot
P90245, POL1_BAMMN;  Q04574, POL1_BAYMG;  Q01206, POL1_BAYMJ;  
Q9YJW3, POL1_BAYMY;  Q65399, POLG_BCMNN;  Q65730, POLG_BSTV1;  
Q65729, POLG_BSTVG;  Q6XW15, POLG_BTMV;  A0AUJ5, POLG_BVY3;  
P17765, POLG_BYMV;  P31999, POLG_LMV0;  P89876, POLG_LMVE;  
P20234, POLG_OMV;  Q01500, POLG_PEMVC;  O56075, POLG_PEMVM;  
P13529, POLG_PPVD;  Q01681, POLG_PPVEA;  P17766, POLG_PPVNA;  
P17767, POLG_PPVRA;  Q84934, POLG_PPVSK;  Q01901, POLG_PRSVH;  
P29152, POLG_PSBMV;  Q85197, POLG_PVMA;  Q02963, POLG_PVYHU;  
P18247, POLG_PVYN;  O11436, POLG_RGMVD;  Q90069, POLG_SBMVG;  
P21231, POLG_SBMVN;  P89201, POLG_SPMMV;  P04517, POLG_TEV;  
A4KZ49, POLG_TRMVU;  P89509, POLG_TUMVJ;  Q02597, POLG_TUMVQ;  
P09814, POLG_TVMV;  P18478, POLG_WMV2U;  P18479, POLG_ZYMVC;  
Q89330, POLG_ZYMVR;  O36979, POLG_ZYMVS;  

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