ID 3.4.22.44 DE nuclear-inclusion-a endopeptidase. AN potyvirus NIa protease. CA Hydrolyzes glutaminyl bonds, and activity is further restricted by CA preferences for the amino acids in P6 - P1' that vary with the species of CA potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme CA from tobacco etch virus. The natural substrate is the viral polyprotein, CA but other proteins and oligopeptides containing the appropriate consensus CA sequence are also cleaved. CC -!- The potyviruses cause diseases in plants, and inclusion bodies appear CC in the host cell nuclei, protein a of these being the endopeptidase. CC -!- The enzyme finds practical use when encoded in vectors for the CC artificial expression of recombinant fusion proteins, since it can CC confer on them the capacity for autolytic cleavage. CC -!- It is also reported that transgenic plants expressing the enzyme are CC resistant to viral infection. CC -!- Belongs to peptidase family C4. DR P90245, POL1_BAMMN ; Q04574, POL1_BAYMG ; Q01206, POL1_BAYMJ ; DR Q9YJW3, POL1_BAYMY ; Q65399, POLG_BCMNN ; Q65730, POLG_BSTV1 ; DR Q65729, POLG_BSTVG ; Q6XW15, POLG_BTMV ; A0AUJ5, POLG_BVY3 ; DR P17765, POLG_BYMV ; P31999, POLG_LMV0 ; P89876, POLG_LMVE ; DR P20234, POLG_OMV ; Q01500, POLG_PEMVC ; O56075, POLG_PEMVM ; DR P13529, POLG_PPVD ; Q01681, POLG_PPVEA ; P17766, POLG_PPVNA ; DR P17767, POLG_PPVRA ; Q84934, POLG_PPVSK ; Q01901, POLG_PRSVH ; DR P29152, POLG_PSBMV ; Q85197, POLG_PVMA ; Q02963, POLG_PVYHU ; DR P18247, POLG_PVYN ; O11436, POLG_RGMVD ; Q90069, POLG_SBMVG ; DR P21231, POLG_SBMVN ; P89201, POLG_SPMMV ; P04517, POLG_TEV ; DR A4KZ49, POLG_TRMVU ; P89509, POLG_TUMVJ ; Q02597, POLG_TUMVQ ; DR P09814, POLG_TVMV ; P18478, POLG_WMV2U ; P18479, POLG_ZYMVC ; DR Q89330, POLG_ZYMVR ; O36979, POLG_ZYMVS ; //