ID 3.4.23.51 DE HycI peptidase. CA This enzyme specifically removes a 32-amino acid peptide from the CA C-terminus of the precursor of the large subunit of E.coli hydrogenase 3 CA by cleavage at the C-terminal side of Arg-537. CC -!- The reaction requires nickel to be bound to the precursor of the CC large subunit of hydrogenase 3. CC -!- The endopeptidase uses the metal in the large subunit of [NiFe]- CC hydrogenases as a recognition motif. CC -!- Belongs to peptidase family A31. DR P0AEW0, HYCI_ECO57 ; P0AEV9, HYCI_ECOLI ; //