ID 3.4.24.55 DE pitrilysin. AN protease III. AN protease Pi. CA Preferential cleavage of 16-Tyr-|-Leu-17 and 25-Phe-|-Tyr-26 bonds of CA oxidized insulin B chain. Also acts on other substrates of Mw less than 7 CA kDa such as insulin and glucagon. CC -!- From the periplasmic space of Escherichia coli. CC -!- Inhibited by EDTA and 1,10-phenanthroline; not thiol dependent. CC -!- Belongs to peptidase family M16. CC -!- Formerly EC 3.4.99.44. DR Q8X6M8, PTRA_ECO57 ; Q8CVS2, PTRA_ECOL6 ; P05458, PTRA_ECOLI ; DR Q8Z418, PTRA_SALTI ; Q8ZMB5, PTRA_SALTY ; Q83QC3, PTRA_SHIFL ; //