ENZYME entry: EC 3.5.1.119

Accepted Name
Pup amidohydrolase.
Alternative Name(s)
Depupylase.
Depupylase/deamidase.
DPUP.
Reaction catalysed
[Prokaryotic ubiquitin-like protein]-L-glutamine + H(2)O <=> [prokaryotic ubiquitin-like protein]-L-glutamate + NH(3)
Comment(s)
  • The enzyme has been characterized from the bacterium Mycobacterium tuberculosis.
  • It catalyzes the hydrolysis of the amido group of the C-terminal glutamine of prokaryotic ubiquitin-like protein (Pup), thus activating it for ligation to target proteins, a process catalyzed by EC 6.3.1.19.
  • Deamination requires ATP as cofactor but not its hydrolysis.
  • The enzyme also catalyzes the hydrolytic cleavage of the bond formed by the ligase, between an epsilon-amino group of a lysine residue of the target protein and the gamma-carboxylate of the C-terminal glutamate of the prokaryotic ubiquitin-like protein.
Cross-references
BRENDA3.5.1.119
EC2PDB3.5.1.119
ExplorEnz3.5.1.119
PRIAM enzyme-specific profiles3.5.1.119
KEGG Ligand Database for Enzyme Nomenclature3.5.1.119
IUBMB Enzyme Nomenclature3.5.1.119
IntEnz3.5.1.119
MEDLINEFind literature relating to 3.5.1.119
MetaCyc3.5.1.119
UniProtKB/Swiss-Prot
A0QZ49, DOP_MYCS2;  P9WNU8, DOP_MYCTO;  P9WNU9, DOP_MYCTU;  
Q53081, DOP_RHOER;  

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.5.1.-
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