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ENZYME

ENZYME entry: EC 3.6.1.71

Accepted Name
adenosine-5'-diphospho-5'-[DNA] diphosphatase
Alternative Name(s)
5'-App5'-DNA adenylate hydrolase
aprataxin
Reaction catalysed
  • a 5'-end adenosine-5'-diphospho-5'-(2'-deoxyribonucleoside)-DNA + H2O <=> a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + AMP + 2 H(+)
  • a 5'-end adenosine-5'-diphospho-5'-ribonucleoside-2'-deoxyribonucleotide-DNA + H2O <=> a 5'-end 5'-monophospho-ribonucleoside-2'-deoxyribonucleotide-DNA + AMP + 2 H(+)
Comment(s)
  • Aprataxin is a DNA-binding protein involved in different types of DNA break repair.
  • The enzyme acts (among other activities) on abortive DNA ligation intermediates that contain an adenylate covalently linked to the 5'-phosphate DNA terminus.
  • It also acts when the adenylate is covalently linked to the 5'-phosphate of a ribonucleotide linked to a DNA strand, which is the result of abortive ligase activty on products of EC 3.1.26.4, an enzyme that cleaves RNA-DNA hybrids on the 5' side of the ribonucleotide found in the 5'-RNA-DNA-3' junction.
  • Aprataxin binds the adenylate group to a histidine residue within the active site, followed by its hydrolysis from the nucleic acid and eventual release, leaving a 5'-phosphate terminus that can be efficiently rejoined.
  • The enzyme also possesses the activities of EC 3.6.1.70 and EC 3.6.1.72.
  • Formerly EC 3.1.11.7.
Cross-references
BRENDA3.6.1.71
EC2PDB3.6.1.71
ExplorEnz3.6.1.71
PRIAM enzyme-specific profiles3.6.1.71
KEGG Ligand Database for Enzyme Nomenclature3.6.1.71
IUBMB Enzyme Nomenclature3.6.1.71
IntEnz3.6.1.71
MEDLINEFind literature relating to 3.6.1.71
MetaCyc3.6.1.71
Rhea expert-curated reactions3.6.1.71
UniProtKB/Swiss-Prot
Q7YRZ2, APTX_BOVINP61797, APTX_CANLFP61798, APTX_CHICK
P61802, APTX_CIOINP61799, APTX_DANREQ7Z2E3, APTX_HUMAN
Q9BGQ0, APTX_MACFAQ7TQC5, APTX_MOUSEQ7YRZ1, APTX_PIG
Q8K4H4, APTX_RATO74859, APTX_SCHPOP61800, APTX_TAKRU
Q7T287, APTX_XENLAP61801, APTX_XENTRQ08702, APTX_YEAST

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.6.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.6.-.-
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