ENZYME entry: EC 4.1.3.44

Accepted Name
tRNA 4-demethylwyosine synthase (AdoMet-dependent).
Reaction catalysed
N(1)-methylguanine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine <=> 4-demethylwyosine(37) in tRNA(Phe) + L-methionine + 5'-deoxyadenosine + CO(2) + H(2)O
Cofactor(s)
Iron-sulfur.
Comment(s)
  • This enzyme, which is a member of the superfamily of S-adenosyl-L- methionine-dependent radical (radical AdoMet) enzymes, binds two [4Fe-4S] clusters.
  • Carbons C(2) and C(3) from pyruvate are incorporated into 4-demethylwyosine.
  • The enzyme is found in eukaryotes where it is part of the pathway for wybutosine synthesis, and in archaea, where it is involved in the biosynthesis of archaeal wye bases, such as wyosine, isowyosine, and methylwyosine.
Cross-references
BRENDA4.1.3.44
EC2PDB4.1.3.44
ExplorEnz4.1.3.44
PRIAM enzyme-specific profiles4.1.3.44
KEGG Ligand Database for Enzyme Nomenclature4.1.3.44
IUBMB Enzyme Nomenclature4.1.3.44
IntEnz4.1.3.44
MEDLINEFind literature relating to 4.1.3.44
MetaCyc4.1.3.44
UniProtKB/Swiss-Prot
Q6NUM6, TYW1B_HUMAN;  Q8RXN5, TYW1_ARATH;  Q08C92, TYW1_DANRE;  
Q9NV66, TYW1_HUMAN;  Q57705, TYW1_METJA;  Q8BJM7, TYW1_MOUSE;  
Q8H8N3, TYW1_ORYSJ;  Q5REF9, TYW1_PONAB;  Q9V1F9, TYW1_PYRAB;  
O59412, TYW1_PYRHO;  O59761, TYW1_SCHPO;  Q2KHP8, TYW1_XENLA;  
Q08960, TYW1_YEAST;  

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 4.1.3.-
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