ENZYME entry: EC 4.1.99.17

Accepted Name
Phosphomethylpyrimidine synthase.
Reaction catalysed
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine <=> 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
Cofactor(s)
Iron-sulfur.
Comment(s)
  • Binds a [4Fe-4S] cluster that is coordinated by 3 cysteines and an exchangeable S-adenosyl-L-methionine molecule.
  • The first stage of catalysis is reduction of the S-adenosyl-L- methionine to produce L-methionine and a 5'-deoxyadenosin-5'-yl radical that is crucial for the conversion of the substrate.
  • Part of the pathway for thiamine biosynthesis.
Cross-references
BRENDA4.1.99.17
EC2PDB4.1.99.17
ExplorEnz4.1.99.17
PRIAM enzyme-specific profiles4.1.99.17
KEGG Ligand Database for Enzyme Nomenclature4.1.99.17
IUBMB Enzyme Nomenclature4.1.99.17
IntEnz4.1.99.17
MEDLINEFind literature relating to 4.1.99.17
MetaCyc4.1.99.17
UniProtKB/Swiss-Prot
Q39YG0, THIC1_GEOMG;  Q8TPW4, THIC1_METAC;  Q46EW5, THIC1_METBF;  
Q8Q0F4, THIC1_METMA;  Q2NHI8, THIC1_METST;  O27586, THIC1_METTH;  
Q2RIM5, THIC1_MOOTA;  Q3A7P7, THIC1_PELCD;  Q97YJ8, THIC1_SULSO;  
Q39RJ6, THIC2_GEOMG;  Q8TI28, THIC2_METAC;  Q46AN2, THIC2_METBF;  
Q8PY39, THIC2_METMA;  Q2NHG6, THIC2_METST;  O27617, THIC2_METTH;  
Q2RHR5, THIC2_MOOTA;  Q3A2D2, THIC2_PELCD;  Q97X14, THIC2_SULSO;  
Q2RHR4, THIC3_MOOTA;  B0CCG9, THIC_ACAM1;  A1TKD9, THIC_ACIAC;  
Q6FFB6, THIC_ACIAD;  B7H1M6, THIC_ACIB3;  B7I331, THIC_ACIB5;  
B2I1W2, THIC_ACIBC;  B0VMD7, THIC_ACIBS;  A3M1D2, THIC_ACIBT;  
B0VDF1, THIC_ACIBY;  B9MFQ3, THIC_ACIET;  B7J7C4, THIC_ACIF2;  
B5EQ61, THIC_ACIF5;  A1WBZ7, THIC_ACISJ;  B9JMY1, THIC_AGRRK;  
Q8UCC9, THIC_AGRT5;  B9K253, THIC_AGRVS;  B6EGV1, THIC_ALISL;  
A6TVU5, THIC_ALKMQ;  A8MEI9, THIC_ALKOO;  B4S2W4, THIC_ALTMD;  
B8JAC3, THIC_ANAD2;  Q2IID8, THIC_ANADE;  A7HCC3, THIC_ANADF;  
Q5PB42, THIC_ANAMM;  Q2GKB1, THIC_ANAPZ;  B4UDM7, THIC_ANASK;  
Q3MGA3, THIC_ANAVT;  O67259, THIC_AQUAE;  O82392, THIC_ARATH;  
O30259, THIC_ARCFU;  Q5P6L3, THIC_AROAE;  A1K3Q2, THIC_AZOSB;  
A7Z2P5, THIC_BACA2;  C3P178, THIC_BACAA;  C3LEJ7, THIC_BACAC;  
A0RL17, THIC_BACAH;  Q81WY7, THIC_BACAN;  B7JGF0, THIC_BACC0;  
P61423, THIC_BACC1;  B7IPY9, THIC_BACC2;  C1EZL5, THIC_BACC3;  
B7HEM8, THIC_BACC4;  B7HX62, THIC_BACC7;  A7GUZ5, THIC_BACCN;  
B9J5N4, THIC_BACCQ;  Q815D5, THIC_BACCR;  Q631C7, THIC_BACCZ;  
Q5LCA5, THIC_BACFN;  Q64T99, THIC_BACFR;  Q9KBJ4, THIC_BACHD;  
Q6HB59, THIC_BACHK;  Q65M65, THIC_BACLD;  A8FFR8, THIC_BACP2;  
P45740, THIC_BACSU;  Q8AA15, THIC_BACTN;  A9VR68, THIC_BACWK;  
A1US11, THIC_BARBK;  Q6G477, THIC_BARHE;  Q6G099, THIC_BARQU;  
A9IRH7, THIC_BART1;  Q1LU45, THIC_BAUCH;  Q2L083, THIC_BORA1;  
Q7WQM6, THIC_BORBR;  Q7W1Q0, THIC_BORPA;  A9IFG8, THIC_BORPD;  
Q7W0D7, THIC_BORPE;  Q89FP0, THIC_BRADU;  A5EPQ5, THIC_BRASB;  
A4YZQ6, THIC_BRASO;  B1YW60, THIC_BURA4;  Q1BXK3, THIC_BURCA;  
B1JYX0, THIC_BURCC;  A0K648, THIC_BURCH;  B4ED40, THIC_BURCJ;  
Q0BGQ9, THIC_BURCM;  A9ACX9, THIC_BURM1;  A3MIG6, THIC_BURM7;  
A2S3Z6, THIC_BURM9;  Q62FE9, THIC_BURMA;  A1V2S9, THIC_BURMS;  
A3NTK3, THIC_BURP0;  Q3JU20, THIC_BURP1;  A3N7W7, THIC_BURP6;  
B2JJF7, THIC_BURP8;  B2SXY6, THIC_BURPP;  Q63VF5, THIC_BURPS;  
Q39HY9, THIC_BURS3;  Q2SUP3, THIC_BURTA;  A4JCZ0, THIC_BURVG;  
Q13WN4, THIC_BURXL;  A0RN29, THIC_CAMFF;  A7I162, THIC_CAMHC;  
A8FKN8, THIC_CAMJ8;  A7H4T0, THIC_CAMJD;  Q9PI55, THIC_CAMJE;  
A1VYG9, THIC_CAMJJ;  Q5HW14, THIC_CAMJR;  Q3AE33, THIC_CARHZ;  
B8GX84, THIC_CAUCN;  Q9A6Q5, THIC_CAUCR;  B0T2Q1, THIC_CAUSK;  
B3PCK0, THIC_CELJU;  Q3ATM0, THIC_CHLCH;  B3EE09, THIC_CHLL2;  
B3QPT5, THIC_CHLP8;  B3EPH6, THIC_CHLPB;  Q8KCH9, THIC_CHLTE;  
Q7P1H8, THIC_CHRVO;  A8AKS9, THIC_CITK8;  Q97EU2, THIC_CLOAB;  
A7FXG9, THIC_CLOB1;  C3L2Y4, THIC_CLOB6;  A5I5Z5, THIC_CLOBH;  
C1FVP6, THIC_CLOBJ;  B1ILH9, THIC_CLOBK;  A7GHD2, THIC_CLOBL;  
B1KZJ7, THIC_CLOBM;  Q186R0, THIC_CLOD6;  B9E2H5, THIC_CLOK1;  
A5N937, THIC_CLOK5;  A0Q1U9, THIC_CLONN;  Q0TTB7, THIC_CLOP1;  
Q8XMK9, THIC_CLOPE;  Q0SV55, THIC_CLOPS;  Q897B2, THIC_CLOTE;  
Q48A96, THIC_COLP3;  P61424, THIC_CORDI;  Q8FPT3, THIC_COREF;  
A4QDR7, THIC_CORGB;  Q8NQW7, THIC_CORGL;  Q4JVZ0, THIC_CORJK;  
B6J5W3, THIC_COXB1;  B6J1X8, THIC_COXB2;  A9KGN7, THIC_COXBN;  
Q83EJ0, THIC_COXBU;  Q0KF34, THIC_CUPNH;  Q476U6, THIC_CUPPJ;  
B2AGF3, THIC_CUPTR;  B1WW40, THIC_CYAA5;  B8HNW1, THIC_CYAP4;  
B7KDN8, THIC_CYAP7;  B7K508, THIC_CYAP8;  Q478T2, THIC_DECAR;  
Q3Z8E2, THIC_DEHE1;  A5FR94, THIC_DEHSB;  Q3ZXE1, THIC_DEHSC;  
Q1IX15, THIC_DEIGD;  Q9RYX8, THIC_DEIRA;  C0QJ00, THIC_DESAH;  
B1I672, THIC_DESAP;  B8FUD2, THIC_DESHD;  A8ZVP4, THIC_DESOH;  
Q6AK99, THIC_DESPS;  A4J8J1, THIC_DESRM;  A7ZUK9, THIC_ECO24;  
B7UPE9, THIC_ECO27;  B7MIY0, THIC_ECO45;  B7LA88, THIC_ECO55;  
Q8X6X9, THIC_ECO57;  B5Z090, THIC_ECO5E;  B7NRS7, THIC_ECO7I;  
B7MRC0, THIC_ECO81;  B7M7Q3, THIC_ECO8A;  C5A0T5, THIC_ECOBW;  
B1XBZ7, THIC_ECODH;  A8A794, THIC_ECOHS;  A1AIG5, THIC_ECOK1;  
Q0TA71, THIC_ECOL5;  Q8FB77, THIC_ECOL6;  B1IUQ3, THIC_ECOLC;  
P30136, THIC_ECOLI;  B7NFT5, THIC_ECOLU;  B6I5K5, THIC_ECOSE;  
B1LNU6, THIC_ECOSM;  Q3YSI0, THIC_EHRCJ;  Q2GG37, THIC_EHRCR;  
Q5FHG9, THIC_EHRRG;  Q5HBN2, THIC_EHRRW;  Q2N8D9, THIC_ERYLH;  
B7LUK8, THIC_ESCF3;  B0S3A6, THIC_FINM2;  Q2JG22, THIC_FRASC;  
Q8RI60, THIC_FUSNN;  Q5L325, THIC_GEOKA;  P61425, THIC_GEOSL;  
C5D571, THIC_GEOSW;  A4IK74, THIC_GEOTN;  Q7NLK5, THIC_GLOVI;  
Q5FNT7, THIC_GLUOX;  Q2SQL8, THIC_HAHCH;  Q5V2X0, THIC_HALMA;  
B0R400, THIC_HALS3;  Q9HRG2, THIC_HALSA;  Q7VFU4, THIC_HELHP;  
A4G224, THIC_HERAR;  Q5QUD0, THIC_IDILO;  A8A8N6, THIC_IGNH4;  
A6SUX7, THIC_JANMA;  B5XYE4, THIC_KLEP3;  A6TGQ1, THIC_KLEP7;  
Q04VF1, THIC_LEPBJ;  Q04Y23, THIC_LEPBL;  B1Y5I3, THIC_LEPCP;  
Q72NZ8, THIC_LEPIC;  Q8F7G5, THIC_LEPIN;  Q2WAV0, THIC_MAGSA;  
A6UT74, THIC_META3;  A7I8Q1, THIC_METB6;  Q603T9, THIC_METCA;  
Q2FQY7, THIC_METHJ;  Q58432, THIC_METJA;  Q8TZ33, THIC_METKA;  
A2SU66, THIC_METLZ;  A4G002, THIC_METM5;  A9A8A9, THIC_METM6;  
A6VIH6, THIC_METM7;  A3CVT7, THIC_METMJ;  P61428, THIC_METMP;  
B8GKY4, THIC_METPE;  A2SG83, THIC_METPP;  A6URG5, THIC_METVS;  
B0JSK4, THIC_MICAN;  A0QLS0, THIC_MYCA1;  B1MIP8, THIC_MYCA9;  
P66912, THIC_MYCBO;  A1KFP5, THIC_MYCBP;  C1AK99, THIC_MYCBT;  
B8ZU90, THIC_MYCLB;  Q9ZBL0, THIC_MYCLE;  B2HQM5, THIC_MYCMM;  
P61426, THIC_MYCPA;  A5TZE9, THIC_MYCTA;  P9WG78, THIC_MYCTO;  
P9WG79, THIC_MYCTU;  A0PTS1, THIC_MYCUA;  Q1D4L3, THIC_MYXXD;  
Q3IRP7, THIC_NATPD;  B9L811, THIC_NAUPA;  Q5F588, THIC_NEIG1;  
A9M0A4, THIC_NEIM0;  Q9JWF3, THIC_NEIMA;  Q9JXI0, THIC_NEIMB;  
A1KWC0, THIC_NEIMF;  Q0AJI9, THIC_NITEC;  Q82Y50, THIC_NITEU;  
Q1QJD3, THIC_NITHX;  Q2Y6P4, THIC_NITMU;  Q3J7C1, THIC_NITOC;  
A6Q2K1, THIC_NITSB;  Q3SPS0, THIC_NITWN;  Q5YNS3, THIC_NOCFA;  
B2J323, THIC_NOSP7;  Q8YY69, THIC_NOSS1;  Q2G7A9, THIC_NOVAD;  
B6JI86, THIC_OLICO;  A1B684, THIC_PARDP;  A7HV30, THIC_PARL1;  
Q6DAM0, THIC_PECAS;  Q3B2V9, THIC_PELLD;  B4SBF6, THIC_PELPB;  
A5D4K5, THIC_PELTS;  Q7N963, THIC_PHOLL;  Q6LVY0, THIC_PHOPR;  
A1VI67, THIC_POLNA;  Q12HI8, THIC_POLSJ;  B2RH34, THIC_PORG3;  
Q7MT71, THIC_PORGI;  B4S9A9, THIC_PROA2;  A3PFA2, THIC_PROM0;  
A2C559, THIC_PROM1;  A8G7B7, THIC_PROM2;  A2C642, THIC_PROM3;  
A9BD54, THIC_PROM4;  A2BYZ5, THIC_PROM5;  Q318D0, THIC_PROM9;  
Q7V9Q8, THIC_PROMA;  Q7V906, THIC_PROMM;  Q7UZP7, THIC_PROMP;  
A2BTJ3, THIC_PROMS;  Q46IK0, THIC_PROMT;  A4SFM6, THIC_PROVI;  
Q48P32, THIC_PSE14;  A6VD86, THIC_PSEA7;  B7V362, THIC_PSEA8;  
Q02F45, THIC_PSEAB;  Q9HUJ2, THIC_PSEAE;  Q1I419, THIC_PSEE4;  
Q4KJA1, THIC_PSEF5;  Q3IFN9, THIC_PSEHT;  A4XPW1, THIC_PSEMY;  
A5W9V8, THIC_PSEP1;  Q3KJ20, THIC_PSEPF;  B0KL24, THIC_PSEPG;  
Q88DA5, THIC_PSEPK;  B1JAG3, THIC_PSEPW;  Q87VG1, THIC_PSESM;  
Q4ZZ08, THIC_PSEU2;  A4VR38, THIC_PSEU5;  Q4FVJ8, THIC_PSYA2;  
Q1QEM6, THIC_PSYCK;  Q9V0X8, THIC_PYRAB;  Q8ZZC0, THIC_PYRAE;  
Q8U0Q4, THIC_PYRFU;  Q1LS28, THIC_RALME;  Q8Y368, THIC_RALSO;  
Q2K206, THIC_RHIEC;  Q98AZ3, THIC_RHILO;  Q92TI9, THIC_RHIME;  
Q7UP26, THIC_RHOBA;  Q21XR6, THIC_RHOFD;  Q2IYP8, THIC_RHOP2;  
Q07PX1, THIC_RHOP5;  P61427, THIC_RHOPA;  Q216H0, THIC_RHOPB;  
Q133T3, THIC_RHOPS;  B3QFS9, THIC_RHOPT;  Q2RST7, THIC_RHORT;  
Q1GEY0, THIC_RUEST;  A1AW65, THIC_RUTMC;  Q21NU6, THIC_SACD2;  
B5F1H7, THIC_SALA4;  A9MHD6, THIC_SALAR;  Q57H61, THIC_SALCH;  
B5FQK9, THIC_SALDC;  B5QYE9, THIC_SALEP;  B5RFJ0, THIC_SALG2;  
B4TCT3, THIC_SALHS;  B4T0Z6, THIC_SALNS;  Q5PKA6, THIC_SALPA;  
A9N0K6, THIC_SALPB;  C0Q2S6, THIC_SALPC;  B5BJR3, THIC_SALPK;  
B4TQK4, THIC_SALSV;  Q8Z326, THIC_SALTI;  Q9L9I7, THIC_SALTY;  
A1S6Q9, THIC_SHEAM;  Q12NC2, THIC_SHEDO;  B0TRP7, THIC_SHEHH;  
A3QER4, THIC_SHELP;  Q8EED7, THIC_SHEON;  A8H566, THIC_SHEPA;  
A4Y6R2, THIC_SHEPC;  A8FUU9, THIC_SHESH;  B1KHI4, THIC_SHEWM;  
B2TWI1, THIC_SHIB3;  Q31U03, THIC_SHIBS;  Q32AG7, THIC_SHIDS;  
Q0SY06, THIC_SHIF8;  Q83PB8, THIC_SHIFL;  Q3YUZ0, THIC_SHISS;  
Q028Z8, THIC_SOLUE;  Q1GQJ3, THIC_SPHAL;  A5VB82, THIC_SPHWW;  
Q82FI7, THIC_STRAW;  Q9X9U0, THIC_STRCO;  B1VPA3, THIC_STRGG;  
B4SI60, THIC_STRM5;  B2FT70, THIC_STRMK;  Q4JCB9, THIC_SULAC;  
Q30QG8, THIC_SULDN;  C3N4K5, THIC_SULIA;  C4KG47, THIC_SULIK;  
C3MMC3, THIC_SULIL;  C3MX60, THIC_SULIM;  Q96ZH0, THIC_SULTO;  
Q2LSU0, THIC_SYNAS;  Q31P93, THIC_SYNE7;  Q2JY68, THIC_SYNJA;  
Q2JPK6, THIC_SYNJB;  B1XIG4, THIC_SYNP2;  Q5N4X6, THIC_SYNP6;  
A5GI51, THIC_SYNPW;  Q7U9W2, THIC_SYNPX;  A5GQ73, THIC_SYNR3;  
Q0IDV6, THIC_SYNS3;  Q3B0I6, THIC_SYNS9;  Q3AND3, THIC_SYNSC;  
Q0AZ84, THIC_SYNWW;  Q55894, THIC_SYNY3;  C5BTK7, THIC_TERTT;  
Q8DLZ2, THIC_THEEB;  C5A6A9, THIC_THEGJ;  Q5JD26, THIC_THEKO;  
A8F821, THIC_THELT;  Q9WZP5, THIC_THEMA;  B9KAI3, THIC_THENN;  
A5IIZ7, THIC_THEP1;  B0KD66, THIC_THEP3;  B0K5Z9, THIC_THEPX;  
B1LCP2, THIC_THESQ;  Q72KP6, THIC_THET2;  Q5SKG5, THIC_THET8;  
Q8R9P1, THIC_THETN;  B5YJ75, THIC_THEYD;  Q31JC9, THIC_THICR;  
Q3SFG3, THIC_THIDA;  Q111Z9, THIC_TRIEI;  C5CM74, THIC_VARPS;  
A5CX15, THIC_VESOH;  A5F4D2, THIC_VIBC3;  A7N141, THIC_VIBCB;  
Q9KVS8, THIC_VIBCH;  C3LPQ3, THIC_VIBCM;  Q5E8W9, THIC_VIBF1;  
B5FF66, THIC_VIBFM;  Q87KF0, THIC_VIBPA;  B7VMV7, THIC_VIBSL;  
Q8DDL4, THIC_VIBVU;  Q7MGM3, THIC_VIBVY;  Q8D247, THIC_WIGBR;  
Q7M818, THIC_WOLSU;  Q8PH13, THIC_XANAC;  Q3BPK7, THIC_XANC5;  
Q4UYF2, THIC_XANC8;  B0RP37, THIC_XANCB;  Q8P5L9, THIC_XANCP;  
Q2P755, THIC_XANOM;  B2SNG3, THIC_XANOP;  Q5H4C0, THIC_XANOR;  
Q9PC93, THIC_XYLFA;  Q87CY6, THIC_XYLFT;  Q8ZAQ2, THIC_YERPE;  
Q66FP5, THIC_YERPS;  Q5NR58, THIC_ZYMMO;  Q8G7X1, THIEC_BIFLO;  

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