ID 4.2.1.74 DE medium-chain-enoyl-CoA hydratase. AN long-chain-enoyl-CoA hydratase. AN long-chain enoyl coenzyme A hydratase. CA a medium-chain (3S)-3-hydroxyacyl-CoA = a medium-chain (2E)-enoyl-CoA + CA H2O. CC -!- Acts in the reverse direction. CC -!- The best substrate for the porcine enzyme is oct-2-enoyl-CoA. CC -!- Unlike EC 4.2.1.17 does not act on crotonoyl-CoA. //