ENZYME

ENZYME entry: EC 4.6.1.14

Accepted Name

Glycosylphosphatidylinositol diacylglycerol-lyase.

Alternative Name(s)

(Glycosyl)phosphatidylinositol-specific phospholipase C.

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol diacylglycerol- lyase (1,2-cyclic-phosphate-forming).

Glycosyl inositol phospholipid anchor-hydrolyzing enzyme.

Glycosylphosphatidylinositol-phospholipase C.

Glycosylphosphatidylinositol-specific phospholipase C.

GPI-PLC.

GPI-specific phospholipase C.

Variant-surface-glycoprotein phospholipase C.

VSG-lipase.

Reaction catalysed

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol <=> 6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol

Comment(s)

This enzyme is also active when O-4 of the glucosamine is substituted by carrying the oligosaccharide that can link a protein to the structure.
It therefore cleaves proteins from the lipid part of the glycosylphostphatidylinositol (GPI) anchors.
In some cases, the long-chain acyl group at the sn-1 position of glycerol is replaced by an alkyl or alk-1-enyl group.
In other cases, the diacylglycerol is replaced by ceramide.
The only characterized enzyme with this specificity is from Trypanosoma brucei, where the acyl groups are myristoyl, but the function of the trypanosome enzyme is unknown.
Substitution on O-2 of the inositol blocks action of this enzyme.
It is not identical with EC 3.1.4.50.
Formerly EC 3.1.4.47.

Cross-references

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature