ENZYME entry: EC 6.1.1.23

Accepted Name
Aspartate--tRNA(Asn) ligase.
Alternative Name(s)
Nondiscriminating aspartyl-tRNA synthetase.
Reaction catalysed
ATP + L-aspartate + tRNA(Asx) <=> AMP + diphosphate + L-aspartyl-tRNA(Asx)
Comment(s)
  • When this enzyme acts on tRNA(Asp), it catalyzes the same reaction as EC 6.1.1.12.
  • It has, however, diminished discrimination, so that it can also form aspartyl-tRNA(Asn).
  • This relaxation of specificity has been found to result from the absence of a loop in the tRNA that specifically recognizes the third position of the anticodon.
  • This accounts for the ability of this enzyme in, for example, Thermus thermophilus, to recognize both tRNA(Asp) (GUC anticodon) and tRNA(Asn) (GUU anticodon).
  • The aspartate-tRNA(Asn) is not used in protein synthesis until it is converted by EC 6.3.5.6 into asparaginyl-tRNA(Asn).
Cross-references
BRENDA6.1.1.23
EC2PDB6.1.1.23
ExplorEnz6.1.1.23
PRIAM enzyme-specific profiles6.1.1.23
KEGG Ligand Database for Enzyme Nomenclature6.1.1.23
IUBMB Enzyme Nomenclature6.1.1.23
IntEnz6.1.1.23
MEDLINEFind literature relating to 6.1.1.23
MetaCyc6.1.1.23
UniProtKB/Swiss-Prot
Q2LPW5, SYDN1_SYNAS;  Q2LTE0, SYDN2_SYNAS;  B0CF01, SYDND_ACAM1;  
A1TT99, SYDND_ACIAC;  Q6FEH6, SYDND_ACIAD;  B7GWN1, SYDND_ACIB3;  
B7I8M5, SYDND_ACIB5;  B2HYL7, SYDND_ACIBC;  B0VRC7, SYDND_ACIBS;  
A3M8Q6, SYDND_ACIBT;  B0V550, SYDND_ACIBY;  A0LUJ6, SYDND_ACIC1;  
C1FAE8, SYDND_ACIC5;  A5G126, SYDND_ACICJ;  B9ME28, SYDND_ACIET;  
B7J3F7, SYDND_ACIF2;  B5EJL3, SYDND_ACIF5;  A1W4E1, SYDND_ACISJ;  
Q9Y9U7, SYDND_AERPE;  B9JCV9, SYDND_AGRRK;  Q8UG87, SYDND_AGRT5;  
B9JV61, SYDND_AGRVS;  Q0VRK1, SYDND_ALCBS;  Q0A582, SYDND_ALKEH;  
B8JH60, SYDND_ANAD2;  Q2IKX7, SYDND_ANADE;  B9KHS0, SYDND_ANAMF;  
Q2GLD8, SYDND_ANAPZ;  B4UIE3, SYDND_ANASK;  Q3MGL3, SYDND_ANAVT;  
B7GFR1, SYDND_ANOFW;  O67589, SYDND_AQUAE;  A8ESW7, SYDND_ARCB4;  
O29342, SYDND_ARCFU;  Q5P280, SYDND_AROAE;  B8H9C7, SYDND_ARTCA;  
A0JXA1, SYDND_ARTS2;  A8IFX1, SYDND_AZOC5;  A1KAJ9, SYDND_AZOSB;  
C1DRG2, SYDND_AZOVD;  A7Z750, SYDND_BACA2;  C3P990, SYDND_BACAA;  
C3L608, SYDND_BACAC;  A0RJ11, SYDND_BACAH;  Q81LI7, SYDND_BACAN;  
B7JPY8, SYDND_BACC0;  Q730D1, SYDND_BACC1;  B7IIR4, SYDND_BACC2;  
C1ESU7, SYDND_BACC3;  B7HE37, SYDND_BACC4;  B7HQG0, SYDND_BACC7;  
A7GT84, SYDND_BACCN;  B9IYX5, SYDND_BACCQ;  Q817X8, SYDND_BACCR;  
Q634E3, SYDND_BACCZ;  Q6HDC3, SYDND_BACHK;  O32038, SYDND_BACSU;  
A9VIM8, SYDND_BACWK;  A1UT52, SYDND_BARBK;  Q6G330, SYDND_BARHE;  
Q6FZI4, SYDND_BARQU;  A9IVI2, SYDND_BART1;  Q6MI59, SYDND_BDEBA;  
B2IKA4, SYDND_BEII9;  C5C5S1, SYDND_BEUC1;  B3DPM0, SYDND_BIFLD;  
Q8G863, SYDND_BIFLO;  B7GPT0, SYDND_BIFLS;  Q2L1K6, SYDND_BORA1;  
Q0SN63, SYDND_BORAP;  Q7WR42, SYDND_BORBR;  O51402, SYDND_BORBU;  
B7J213, SYDND_BORBZ;  B5RM04, SYDND_BORDL;  Q661H4, SYDND_BORGA;  
B2S0F0, SYDND_BORHD;  Q7W275, SYDND_BORPA;  A9IHE2, SYDND_BORPD;  
Q7W001, SYDND_BORPE;  B5RRQ0, SYDND_BORRA;  A1QZN3, SYDND_BORT9;  
Q89MP9, SYDND_BRADU;  C0R2C9, SYDND_BRAHW;  A5EIE6, SYDND_BRASB;  
A4YTC4, SYDND_BRASO;  B2S501, SYDND_BRUA1;  Q2YNA7, SYDND_BRUA2;  
Q57DZ8, SYDND_BRUAB;  A9MAD3, SYDND_BRUC2;  C0RI89, SYDND_BRUMB;  
P67012, SYDND_BRUME;  A5VPT5, SYDND_BRUO2;  B0CL77, SYDND_BRUSI;  
P67013, SYDND_BRUSU;  B1YWF2, SYDND_BURA4;  Q1BTP1, SYDND_BURCA;  
B1JYI9, SYDND_BURCC;  A0KAE8, SYDND_BURCH;  B4EBD1, SYDND_BURCJ;  
Q0BBZ1, SYDND_BURCM;  A9AFC7, SYDND_BURM1;  A3MNU6, SYDND_BURM7;  
A2S8L9, SYDND_BURM9;  Q62MP0, SYDND_BURMA;  A1V745, SYDND_BURMS;  
A3NRK2, SYDND_BURP0;  Q3JVY8, SYDND_BURP1;  A3N5V6, SYDND_BURP6;  
B2JCV5, SYDND_BURP8;  B2SX42, SYDND_BURPP;  Q63X93, SYDND_BURPS;  
Q39D20, SYDND_BURS3;  Q2T132, SYDND_BURTA;  A4JHR9, SYDND_BURVG;  
Q145N3, SYDND_BURXL;  Q8RAI7, SYDND_CALS4;  A7ZEH3, SYDND_CAMC1;  
A0RQ71, SYDND_CAMFF;  A7I058, SYDND_CAMHC;  A8FL61, SYDND_CAMJ8;  
A7H4H2, SYDND_CAMJD;  Q9PHM7, SYDND_CAMJE;  A1VZ00, SYDND_CAMJJ;  
Q5HVD1, SYDND_CAMJR;  Q3AA17, SYDND_CARHZ;  Q9A734, SYDND_CAUCR;  
B0SWQ6, SYDND_CAUSK;  B3PB55, SYDND_CELJU;  Q11JA7, SYDND_CHESB;  
A9WGJ7, SYDND_CHLAA;  Q5L734, SYDND_CHLAB;  B8G6W9, SYDND_CHLAD;  
Q3AS30, SYDND_CHLCH;  Q46175, SYDND_CHLCV;  B3EDE5, SYDND_CHLL2;  
Q9PJK0, SYDND_CHLMU;  B3QP90, SYDND_CHLP8;  B3EQ26, SYDND_CHLPB;  
A1BGZ4, SYDND_CHLPD;  Q9Z7P2, SYDND_CHLPN;  B9LJ78, SYDND_CHLSY;  
B0B8B6, SYDND_CHLT2;  Q3KLF5, SYDND_CHLTA;  B0B9Z5, SYDND_CHLTB;  
Q8KCT7, SYDND_CHLTE;  O84546, SYDND_CHLTR;  Q1QWG2, SYDND_CHRSD;  
Q7NRP0, SYDND_CHRVO;  A9KIA6, SYDND_CLOPH;  Q483C7, SYDND_COLP3;  
C3PGN5, SYDND_CORA7;  Q6NGZ4, SYDND_CORDI;  Q8FT19, SYDND_COREF;  
A4QEL0, SYDND_CORGB;  Q8NQ19, SYDND_CORGL;  Q4JVF2, SYDND_CORJK;  
C4LIW2, SYDND_CORK4;  B1VDL1, SYDND_CORU7;  A9KF44, SYDND_COXBN;  
A9N999, SYDND_COXBR;  Q83BE5, SYDND_COXBU;  Q0KEG8, SYDND_CUPNH;  
Q475W2, SYDND_CUPPJ;  B2AH15, SYDND_CUPTR;  B1WRQ9, SYDND_CYAA5;  
B8HXQ8, SYDND_CYAP4;  B7KB89, SYDND_CYAP7;  B7JX16, SYDND_CYAP8;  
Q47IE0, SYDND_DECAR;  Q3Z8K0, SYDND_DEHM1;  A5FRE3, SYDND_DEHMB;  
Q3ZX84, SYDND_DEHMC;  Q9RVH4, SYDND_DEIRA;  A9BP37, SYDND_DELAS;  
B8FKS6, SYDND_DESAA;  Q317R3, SYDND_DESAG;  C0QFI1, SYDND_DESAH;  
A8ZUR7, SYDND_DESOH;  Q6AQS4, SYDND_DESPS;  A4J2J2, SYDND_DESRM;  
Q725Q7, SYDND_DESVH;  B5YDT0, SYDND_DICT6;  B8E1C0, SYDND_DICTD;  
A8LI32, SYDND_DINSH;  Q3YRF1, SYDND_EHRCJ;  Q2GHC9, SYDND_EHRCR;  
Q5FG09, SYDND_EHRRG;  Q5HAL5, SYDND_EHRRW;  B2KBB9, SYDND_ELUMP;  
Q2NBQ6, SYDND_ERYLH;  B0TW08, SYDND_FRAP2;  A7N949, SYDND_FRATF;  
Q2A622, SYDND_FRATH;  Q0BPA2, SYDND_FRATO;  Q5NIQ4, SYDND_FRATT;  
A4J0C7, SYDND_FRATW;  B5EIV1, SYDND_GEOBB;  B9M1C9, SYDND_GEODF;  
Q39VY3, SYDND_GEOMG;  Q74D56, SYDND_GEOSL;  C6E3W7, SYDND_GEOSM;  
C5D509, SYDND_GEOSW;  A5G3L5, SYDND_GEOUR;  Q7NJA4, SYDND_GLOVI;  
Q5FQ60, SYDND_GLUOX;  Q2SCK8, SYDND_HAHCH;  Q5V1N2, SYDND_HALMA;  
B8CXE5, SYDND_HALOH;  O07683, SYDND_HALSA;  O24822, SYDND_HALVO;  
Q18KP5, SYDND_HALWD;  Q17XK9, SYDND_HELAH;  Q7VF76, SYDND_HELHP;  
B0TF89, SYDND_HELMI;  B6JLK1, SYDND_HELP2;  B5Z6Y8, SYDND_HELPG;  
Q1CTQ7, SYDND_HELPH;  Q9ZLL9, SYDND_HELPJ;  B2UTL0, SYDND_HELPS;  
P56459, SYDND_HELPY;  A9B392, SYDND_HERA2;  A4G8X1, SYDND_HERAR;  
B4U7P3, SYDND_HYDS0;  Q0BZZ1, SYDND_HYPNA;  Q28MH9, SYDND_JANSC;  
A6WCG5, SYDND_KINRD;  C1DB91, SYDND_LARHH;  Q5X586, SYDND_LEGPA;  
A5IBW8, SYDND_LEGPC;  Q5ZVG2, SYDND_LEGPH;  Q5WWA3, SYDND_LEGPL;  
B1XWR8, SYDND_LEPCP;  Q2W3D5, SYDND_MAGSA;  A0L667, SYDND_MAGSM;  
A1U1C2, SYDND_MARHV;  Q0AQ87, SYDND_MARMM;  A6VXD0, SYDND_MARMS;  
A6USZ9, SYDND_META3;  Q8TQ68, SYDND_METAC;  Q466Y5, SYDND_METBF;  
Q12XU7, SYDND_METBU;  B7KN33, SYDND_METC4;  Q607I4, SYDND_METCA;  
Q1GYN8, SYDND_METFK;  Q2FRU5, SYDND_METHJ;  B3DX01, SYDND_METI4;  
Q58950, SYDND_METJA;  Q8TXG4, SYDND_METKA;  A2SPG1, SYDND_METLZ;  
A4G0V5, SYDND_METM5;  A9A945, SYDND_METM6;  A6VHK5, SYDND_METM7;  
Q8Q0R2, SYDND_METMA;  A3CXH4, SYDND_METMJ;  Q6LWU0, SYDND_METMP;  
B8IUT2, SYDND_METNO;  B1ZFX6, SYDND_METPB;  A2SEP2, SYDND_METPP;  
B0UI66, SYDND_METS4;  B8EN55, SYDND_METSB;  Q2NHT0, SYDND_METST;  
O26328, SYDND_METTH;  A6UQQ8, SYDND_METVS;  B0JNW9, SYDND_MICAN;  
C5CCH2, SYDND_MICLC;  Q2RHW1, SYDND_MOOTA;  A0QI91, SYDND_MYCA1;  
B1MCH3, SYDND_MYCA9;  Q7TYA6, SYDND_MYCBO;  A4TBU4, SYDND_MYCGI;  
B8ZUJ3, SYDND_MYCLB;  P36429, SYDND_MYCLE;  Q741M7, SYDND_MYCPA;  
A0QWN3, SYDND_MYCS2;  A3PZ05, SYDND_MYCSJ;  A1UFE5, SYDND_MYCSK;  
P9WFW2, SYDND_MYCTO;  P9WFW3, SYDND_MYCTU;  Q3IT59, SYDND_NATPD;  
B2A2G0, SYDND_NATTJ;  B4RPA1, SYDND_NEIG2;  A9M257, SYDND_NEIM0;  
Q9JT23, SYDND_NEIMA;  Q9K0U5, SYDND_NEIMB;  A1KVG2, SYDND_NEIMF;  
Q0AIE4, SYDND_NITEC;  Q82SQ5, SYDND_NITEU;  Q1QLI9, SYDND_NITHX;  
Q2YBG1, SYDND_NITMU;  Q3JEB6, SYDND_NITOC;  Q3SS92, SYDND_NITWN;  
Q5YTJ4, SYDND_NOCFA;  A1SJB8, SYDND_NOCSJ;  B2J116, SYDND_NOSP7;  
Q8YUB6, SYDND_NOSS1;  Q2G8P1, SYDND_NOVAD;  A6X200, SYDND_OCHA4;  
B6JGJ7, SYDND_OLICO;  B1ZSW8, SYDND_OPITP;  A5CC91, SYDND_ORITB;  
B3CV53, SYDND_ORITI;  A1B431, SYDND_PARDP;  A7HYC4, SYDND_PARL1;  
Q6ME91, SYDND_PARUW;  Q3A5R7, SYDND_PELCD;  Q3B3A1, SYDND_PELLD;  
B4SAH1, SYDND_PELPB;  A5D3E3, SYDND_PELTS;  Q4FMP8, SYDND_PELUB;  
C0QT74, SYDND_PERMH;  B4RCT1, SYDND_PHEZH;  B4S8V2, SYDND_PROA2;  
Q6A8J1, SYDND_PROAC;  A3PFH7, SYDND_PROM0;  A8G7J3, SYDND_PROM2;  
A2CDX5, SYDND_PROM3;  A9BDD8, SYDND_PROM4;  A2BZ75, SYDND_PROM5;  
Q317V3, SYDND_PROM9;  Q7V9I1, SYDND_PROMA;  Q7V3X1, SYDND_PROMM;  
Q7UZH8, SYDND_PROMP;  A2BTS0, SYDND_PROMS;  Q46I98, SYDND_PROMT;  
A4SF07, SYDND_PROVI;  Q48FC1, SYDND_PSE14;  Q15RN2, SYDND_PSEA6;  
B7UXW9, SYDND_PSEA8;  Q02IC5, SYDND_PSEAB;  Q51422, SYDND_PSEAE;  
Q1I6E5, SYDND_PSEE4;  Q4K7D5, SYDND_PSEF5;  C3JYT1, SYDND_PSEFS;  
A4XRR7, SYDND_PSEMY;  A5VZU3, SYDND_PSEP1;  Q3K7V6, SYDND_PSEPF;  
B0KTJ6, SYDND_PSEPG;  Q88NJ4, SYDND_PSEPK;  B1JD74, SYDND_PSEPW;  
Q87Y31, SYDND_PSESM;  Q4ZWL5, SYDND_PSEU2;  Q4FSF4, SYDND_PSYA2;  
Q1QBI9, SYDND_PSYCK;  A1SST6, SYDND_PSYIN;  A5WE50, SYDND_PSYWF;  
Q1LRG2, SYDND_RALME;  Q8Y270, SYDND_RALSO;  Q1MIW0, SYDND_RHIL3;  
Q983E9, SYDND_RHILO;  B5ZX21, SYDND_RHILW;  Q92QV4, SYDND_RHIME;  
C3M9G8, SYDND_RHISN;  Q7UFY6, SYDND_RHOBA;  B6IN46, SYDND_RHOCS;  
C0ZZA3, SYDND_RHOE4;  Q21YX2, SYDND_RHOFT;  C1B4G8, SYDND_RHOOB;  
Q2IX59, SYDND_RHOP2;  Q07LH5, SYDND_RHOP5;  Q6N5D6, SYDND_RHOPA;  
Q215P7, SYDND_RHOPB;  Q135R5, SYDND_RHOPS;  B3Q9Q1, SYDND_RHOPT;  
Q2RSM3, SYDND_RHORT;  A3PMK8, SYDND_RHOS1;  Q3IZP1, SYDND_RHOS4;  
A4WPF7, SYDND_RHOS5;  B9KME7, SYDND_RHOSK;  Q0S0P3, SYDND_RHOSR;  
C3PMI3, SYDND_RICAE;  A8GMB3, SYDND_RICAH;  A8GXC5, SYDND_RICB8;  
Q1RJN4, SYDND_RICBR;  A8EXL4, SYDND_RICCK;  Q92J82, SYDND_RICCN;  
Q4UKE2, SYDND_RICFE;  Q9ZE17, SYDND_RICPR;  Q68XM3, SYDND_RICTY;  
A7NQH9, SYDND_ROSCS;  Q16B16, SYDND_ROSDO;  A5UUR3, SYDND_ROSS1;  
Q1AWB4, SYDND_RUBXD;  Q5LUX6, SYDND_RUEPO;  Q1GIZ8, SYDND_RUEST;  
A1AW56, SYDND_RUTMC;  Q21MA4, SYDND_SACD2;  A4FBC3, SYDND_SACEN;  
A8LXY1, SYDND_SALAI;  Q2S158, SYDND_SALRD;  A4X5Y3, SYDND_SALTO;  
A6U7M8, SYDND_SINMW;  Q8DSG3, SYDND_STRMU;  Q30RL7, SYDND_SULDN;  
A6QAE8, SYDND_SULNB;  Q980V3, SYDND_SULSO;  B2V713, SYDND_SULSY;  
Q976I3, SYDND_SULTO;  Q67LP0, SYDND_SYMTH;  Q31NM6, SYDND_SYNE7;  
Q2JTZ2, SYDND_SYNJA;  Q2JMA8, SYDND_SYNJB;  B1XNY9, SYDND_SYNP2;  
Q5N5I8, SYDND_SYNP6;  A5GPL9, SYDND_SYNPW;  Q7U3K6, SYDND_SYNPX;  
A5GWT2, SYDND_SYNR3;  Q3AVS9, SYDND_SYNS9;  Q3AGF9, SYDND_SYNSC;  
Q0AYS1, SYDND_SYNWW;  P73851, SYDND_SYNY3;  C5BPP4, SYDND_TERTT;  
Q8DJS8, SYDND_THEEB;  Q47N50, SYDND_THEFY;  Q9X1F4, SYDND_THEMA;  
B9K8E5, SYDND_THENN;  B0K978, SYDND_THEP3;  B0K0N5, SYDND_THEPX;  
B1LBH9, SYDND_THESQ;  Q5SIC2, SYDND_THET8;  B5YJP5, SYDND_THEYD;  
Q31H87, SYDND_THICR;  Q3SLL2, SYDND_THIDA;  B8GUK0, SYDND_THISH;  
Q73MC2, SYDND_TREDE;  O83950, SYDND_TREPA;  B2S4M2, SYDND_TREPS;  
Q112K0, SYDND_TRIEI;  B1GZY6, SYDND_UNCTG;  C5CWX8, SYDND_VARPS;  
A1WHI6, SYDND_VEREI;  Q73HX6, SYDND_WOLPM;  B3CPJ5, SYDND_WOLPP;  
Q7M8A6, SYDND_WOLSU;  Q5GTS0, SYDND_WOLTR;  C0R2K4, SYDND_WOLWR;  
A7IK27, SYDND_XANP2;  Q5NPM1, SYDND_ZYMMO;  

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