ENZYME entry: EC 6.3.5.11
| Accepted Name |
|---|
| Cobyrinate a,c-diamide synthase (glutamine-hydrolyzing).
|
| Alternative Name(s) |
|---|
| Cobyrinic acid a,c-diamide synthetase. |
| Reaction catalysed |
|---|
| 2 ATP + cobyrinate + 2 L-glutamine + 2 H(2)O <=> 2 ADP + 2 phosphate + cobyrinate a,c-diamide + 2 L-glutamate |
| Comment(s) |
|---|
- This enzyme is the first glutamine amidotransferase that participates
in the anaerobic (early cobalt insertion) biosynthetic pathway of
adenosylcobalamin, and catalyzes the ATP-dependent synthesis of
cobyrinate a,c-diamide from cobyrinate using either L-glutamine or
ammonia as the nitrogen source.
- It is proposed that the enzyme first catalyzes the amidation of the
c-carboxylate, and then the intermediate is released into solution
and binds to the same catalytic site for the amidation of the
a-carboxylate.
- The Km for ammonia is substantially higher than that for L-glutamine.
|
| Cross-references |
|---|
| BRENDA | 6.3.5.11 |
| EC2PDB | 6.3.5.11 |
| ExplorEnz | 6.3.5.11 |
| PRIAM enzyme-specific profiles | 6.3.5.11 |
| KEGG Ligand Database for Enzyme Nomenclature | 6.3.5.11 |
| IUBMB Enzyme Nomenclature | 6.3.5.11 |
| IntEnz | 6.3.5.11 |
| MEDLINE | Find literature relating to 6.3.5.11 |
| MetaCyc | 6.3.5.11 |
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