ENZYME entry: EC 6.3.5.11

Accepted Name
Cobyrinate a,c-diamide synthase (glutamine-hydrolyzing).
Alternative Name(s)
Cobyrinic acid a,c-diamide synthetase.
Reaction catalysed
2 ATP + cobyrinate + 2 L-glutamine + 2 H(2)O <=> 2 ADP + 2 phosphate + cobyrinate a,c-diamide + 2 L-glutamate
Comment(s)
  • This enzyme is the first glutamine amidotransferase that participates in the anaerobic (early cobalt insertion) biosynthetic pathway of adenosylcobalamin, and catalyzes the ATP-dependent synthesis of cobyrinate a,c-diamide from cobyrinate using either L-glutamine or ammonia as the nitrogen source.
  • It is proposed that the enzyme first catalyzes the amidation of the c-carboxylate, and then the intermediate is released into solution and binds to the same catalytic site for the amidation of the a-carboxylate.
  • The Km for ammonia is substantially higher than that for L-glutamine.
Cross-references
BRENDA6.3.5.11
EC2PDB6.3.5.11
ExplorEnz6.3.5.11
PRIAM enzyme-specific profiles6.3.5.11
KEGG Ligand Database for Enzyme Nomenclature6.3.5.11
IUBMB Enzyme Nomenclature6.3.5.11
IntEnz6.3.5.11
MEDLINEFind literature relating to 6.3.5.11
MetaCyc6.3.5.11
UniProtKB/Swiss-Prot
O28054, CBIA_ARCFU;  Q9KBM8, CBIA_BACHD;  O87698, CBIA_BACME;  
Q8RCP4, CBIA_CALS4;  Q97JB1, CBIA_CLOAB;  Q897K3, CBIA_CLOTE;  
Q3ZA71, CBIA_DEHM1;  Q3ZWJ9, CBIA_DEHMC;  Q8R659, CBIA_FUSNN;  
Q7NF32, CBIA_GLOVI;  Q5V341, CBIA_HALMA;  Q9HPL7, CBIA_HALSA;  
A4G3R1, CBIA_HERAR;  A5VM87, CBIA_LACRD;  B2G9J4, CBIA_LACRJ;  
Q75FQ8, CBIA_LEPIC;  Q8EXQ4, CBIA_LEPIN;  Q92CL7, CBIA_LISIN;  
Q720N8, CBIA_LISMF;  Q8Y7T0, CBIA_LISMO;  A0L8B9, CBIA_MAGMM;  
Q58816, CBIA_METJA;  A4FW46, CBIA_METM5;  A6VH63, CBIA_METM7;  
A5UMJ2, CBIA_METS3;  Q2NHQ3, CBIA_METST;  O27509, CBIA_METTH;  
A6UQC1, CBIA_METVS;  Q3A7A3, CBIA_PELCD;  A1AT17, CBIA_PELPD;  
Q6L2V8, CBIA_PICTO;  P29946, CBIA_SALTY;  A3CL56, CBIA_STRSV;  
Q4JAI7, CBIA_SULAC;  Q980B1, CBIA_SULSO;  Q975N0, CBIA_SULTO;  
Q5MZU1, CBIA_SYNP6;  P73002, CBIA_SYNY3;  Q9HIX6, CBIA_THEAC;  
Q97BP4, CBIA_THEVO;  

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