ENZYME entry: EC 6.3.5.5

Accepted Name
Carbamoyl-phosphate synthase (glutamine-hydrolyzing).
Alternative Name(s)
Carbamoyl phosphate synthetase.
Carbamoyl-phosphate synthetase (glutamine-hydrolyzing).
Carbamoylphosphate synthetase II.
Carbamyl phosphate synthetase (glutamine).
CPS.
GD-CPSase.
Glutamine-dependent carbamoyl-phosphate synthase.
Glutamine-dependent carbamyl phosphate synthetase.
Reaction catalysed
2 ATP + L-glutamine + HCO(3)(-) + H(2)O <=> 2 ADP + phosphate + L-glutamate + carbamoyl phosphate
Comment(s)
  • The product carbamoyl phosphate is an intermediate in the biosynthesis of arginine and the pyrimidine nucleotides.
  • The enzyme from Escherichia coli has three separate active sites, which are connected by a molecular tunnel that is almost 100 A in length.
  • The amidotransferase domain within the small subunit of the enzyme hydrolyzes glutamine to ammonia via a thioester intermediate.
  • The ammonia migrates through the interior of the protein, where it reacts with carboxyphosphate to produce the carbamate intermediate.
  • The carboxyphosphate intermediate is formed by the phosphorylation of hydrogencarbonate by ATP at a site contained within the N-terminal half of the large subunit.
  • The carbamate intermediate is transported through the interior of the protein to a second site within the C-terminal half of the large subunit, where it is phosphorylated by another ATP to yield the final product, carbamoyl phosphate.
  • Cf. EC 6.3.4.16.
  • Formerly EC 2.7.2.9.
Cross-references
PROSITEPDOC00405 ; PDOC00676 ; PDOC50975
BRENDA6.3.5.5
EC2PDB6.3.5.5
ExplorEnz6.3.5.5
PRIAM enzyme-specific profiles6.3.5.5
KEGG Ligand Database for Enzyme Nomenclature6.3.5.5
IUBMB Enzyme Nomenclature6.3.5.5
IntEnz6.3.5.5
MEDLINEFind literature relating to 6.3.5.5
MetaCyc6.3.5.5
UniProtKB/Swiss-Prot
Q6F8M7, CARA_ACIAD;  Q8UDF7, CARA_AGRFC;  B2URJ0, CARA_AKKM8;  
O66727, CARA_AQUAE;  Q9LVW7, CARA_ARATH;  O28995, CARA_ARCFU;  
Q752N9, CARA_ASHGO;  A1CA18, CARA_ASPCL;  Q4WU09, CARA_ASPFU;  
A2R9B9, CARA_ASPNC;  Q2U913, CARA_ASPOR;  Q81WF1, CARA_BACAN;  
Q732I2, CARA_BACC1;  P52557, CARA_BACCL;  Q819S2, CARA_BACCR;  
Q636D9, CARA_BACCZ;  Q9K9V8, CARA_BACHD;  Q6HES7, CARA_BACHK;  
P25993, CARA_BACSU;  A1UTE8, CARA_BARBK;  Q6G5M0, CARA_BARHE;  
Q6FZ66, CARA_BARQU;  A9IWF8, CARA_BART1;  B3DQ31, CARA_BIFLD;  
Q8G816, CARA_BIFLO;  B7GPW1, CARA_BIFLS;  Q89DR8, CARA_BRADU;  
B2S6U9, CARA_BRUA1;  Q2YM22, CARA_BRUA2;  Q57C28, CARA_BRUAB;  
A9M6F1, CARA_BRUC2;  C0REC2, CARA_BRUMB;  Q8YIB8, CARA_BRUME;  
A5VRM1, CARA_BRUO2;  B0CHS0, CARA_BRUSI;  Q8FZJ8, CARA_BRUSU;  
P57245, CARA_BUCAI;  Q8K9Z6, CARA_BUCAP;  P59576, CARA_BUCBP;  
Q8RBK1, CARA_CALS4;  Q9PMG8, CARA_CAMJE;  Q5AML6, CARA_CANAL;  
Q6FQ30, CARA_CANGA;  Q9A4J7, CARA_CAUCR;  Q2H132, CARA_CHAGB;  
Q8KGA2, CARA_CHLTE;  Q97FT2, CARA_CLOAB;  Q8XHB2, CARA_CLOPE;  
Q1DUF5, CARA_COCIM;  Q6NH15, CARA_CORDI;  Q8FT41, CARA_COREF;  
P58893, CARA_CORGL;  O19886, CARA_CYACA;  Q6BJG4, CARA_DEBHA;  
Q1IWN0, CARA_DEIGD;  Q9RWI4, CARA_DEIRA;  C6C0A9, CARA_DESAD;  
B8IZS6, CARA_DESDA;  C4XRH8, CARA_DESMR;  Q726J4, CARA_DESVH;  
Q8KZA0, CARA_DESVM;  A1VA26, CARA_DESVV;  P0A6F2, CARA_ECO57;  
Q8FLB1, CARA_ECOL6;  P0A6F1, CARA_ECOLI;  Q5BB37, CARA_EMENI;  
Q8RG87, CARA_FUSNN;  Q6B940, CARA_GRATL;  Q7VP66, CARA_HAEDU;  
Q8RSS4, CARA_HALER;  B0R6F1, CARA_HALS3;  Q9HP42, CARA_HALSA;  
Q17VI1, CARA_HELAH;  Q7VHS5, CARA_HELHP;  Q9ZJY9, CARA_HELPJ;  
O25835, CARA_HELPY;  Q0BY73, CARA_HYPNA;  P87183, CARA_HYPVI;  
Q6CIQ1, CARA_KLULA;  Q9CF80, CARA_LACLA;  Q9L4N5, CARA_LACLM;  
P77885, CARA_LACPL;  Q1MPN3, CARA_LAWIP;  Q04R57, CARA_LEPBJ;  
Q04ZG6, CARA_LEPBL;  Q72PK5, CARA_LEPIC;  Q8F6R2, CARA_LEPIN;  
Q92AH2, CARA_LISIN;  Q71YI0, CARA_LISMF;  Q8Y664, CARA_LISMO;  
Q0AMR3, CARA_MARMM;  Q8TNY3, CARA_METAC;  Q58425, CARA_METJA;  
Q8TY15, CARA_METKA;  A4G0Y3, CARA_METM5;  A9A972, CARA_METM6;  
A6VHH7, CARA_METM7;  Q8Q0U4, CARA_METMA;  Q6LWW5, CARA_METMP;  
A4YI77, CARA_METS5;  O27079, CARA_METTH;  A6UQN4, CARA_METVS;  
Q7U055, CARA_MYCBO;  Q9CCR3, CARA_MYCLE;  Q741H2, CARA_MYCPA;  
P9WPK4, CARA_MYCTO;  P9WPK5, CARA_MYCTU;  Q50983, CARA_NEIGO;  
Q9JVZ6, CARA_NEIMA;  Q9JXX4, CARA_NEIMB;  A1CZ92, CARA_NEOFI;  
P22572, CARA_NEUCR;  Q8YXQ7, CARA_NOSS1;  Q8CXH8, CARA_OCEIH;  
A6WZJ5, CARA_OCHA4;  Q6YV23, CARA_ORYSJ;  Q9CKV3, CARA_PASMU;  
Q6D0C8, CARA_PECAS;  Q3A450, CARA_PELCD;  Q0U5H7, CARA_PHANO;  
Q7N8W2, CARA_PHOLL;  Q6LUK6, CARA_PHOPR;  B1XUM3, CARA_POLNS;  
A4SXM1, CARA_POLSQ;  P51201, CARA_PORPU;  B4S5S1, CARA_PROA2;  
P38098, CARA_PSEAE;  Q88DU5, CARA_PSEPK;  Q87WP3, CARA_PSESM;  
P38099, CARA_PSEST;  Q8ZY49, CARA_PYRAE;  Q8U086, CARA_PYRFU;  
Q1XDT6, CARA_PYRYE;  Q8XZ85, CARA_RALSO;  Q98IA7, CARA_RHILO;  
Q92N95, CARA_RHIME;  Q7UGJ6, CARA_RHOBA;  Q9JP87, CARA_RUBGI;  
Q5LTN6, CARA_RUEPO;  Q8Z9L8, CARA_SALTI;  P14845, CARA_SALTY;  
O60060, CARA_SCHPO;  Q8EHS6, CARA_SHEON;  Q5HGN0, CARA_STAAC;  
P63729, CARA_STAAM;  P99147, CARA_STAAN;  Q6GHN3, CARA_STAAR;  
Q6GA11, CARA_STAAS;  P63730, CARA_STAAW;  Q5HPY9, CARA_STAEQ;  
Q8CPJ5, CARA_STAES;  Q4L5Q4, CARA_STAHJ;  Q49WY3, CARA_STAS1;  
P63731, CARA_STRA3;  P63732, CARA_STRA5;  Q827Q8, CARA_STRAW;  
Q9KXR5, CARA_STRCO;  Q8DUP4, CARA_STRMU;  P63735, CARA_STRP1;  
P0DA12, CARA_STRP3;  Q5XCR8, CARA_STRP6;  P58894, CARA_STRP8;  
P63733, CARA_STRPN;  P0DA13, CARA_STRPQ;  P63734, CARA_STRR6;  
Q4J8E9, CARA_SULAC;  Q59968, CARA_SULSO;  Q970U8, CARA_SULTO;  
Q67Q55, CARA_SYMTH;  Q31LB7, CARA_SYNE7;  Q5N0L0, CARA_SYNP6;  
Q7U7F9, CARA_SYNPX;  A5GTM5, CARA_SYNR3;  P74587, CARA_SYNY3;  
C5BQ30, CARA_TERTT;  Q9HK16, CARA_THEAC;  Q8DKU5, CARA_THEEB;  
Q9WZ28, CARA_THEMA;  Q72GZ0, CARA_THET2;  Q5SLL3, CARA_THET8;  
Q97AJ4, CARA_THEVO;  B8GNX4, CARA_THISH;  Q9KPH8, CARA_VIBCH;  
Q87SF4, CARA_VIBPA;  Q8DEM1, CARA_VIBVU;  Q7MNU1, CARA_VIBVY;  
Q8D3H7, CARA_WIGBR;  P58895, CARA_XANAC;  P58896, CARA_XANCP;  
Q9PEC2, CARA_XYLFA;  Q87EB9, CARA_XYLFT;  Q6C9Y4, CARA_YARLI;  
P07258, CARA_YEAST;  Q8ZIL5, CARA_YERPE;  Q66ER8, CARA_YERPS;  
O50235, CARA_ZYMMO;  O67869, CARB1_AQUAE;  Q58773, CARB1_METJA;  
Q8TWX0, CARB1_METKA;  O67233, CARB2_AQUAE;  Q58776, CARB2_METJA;  
Q8TUT7, CARB2_METKA;  C1F1S6, CARB_ACIC5;  C4ZEK2, CARB_AGARV;  
Q8UDE9, CARB_AGRFC;  Q42601, CARB_ARATH;  O28994, CARB_ARCFU;  
A1R6Z3, CARB_ARTAT;  A0JX72, CARB_ARTS2;  Q75D66, CARB_ASHGO;  
C3P656, CARB_BACAA;  C3L740, CARB_BACAC;  A0RHQ8, CARB_BACAH;  
Q81WF2, CARB_BACAN;  B7JJX3, CARB_BACC0;  Q732I3, CARB_BACC1;  
B7IUP6, CARB_BACC2;  C1EPQ0, CARB_BACC3;  B7H6M2, CARB_BACC4;  
B7HLM0, CARB_BACC7;  P46537, CARB_BACCL;  A7GRL1, CARB_BACCN;  
B9IVW3, CARB_BACCQ;  Q819S3, CARB_BACCR;  Q636E0, CARB_BACCZ;  
Q9K9V9, CARB_BACHD;  Q6HES8, CARB_BACHK;  P25994, CARB_BACSU;  
A9VTC6, CARB_BACWK;  C5C687, CARB_BEUC1;  B8DTW3, CARB_BIFA0;  
A1A0T4, CARB_BIFAA;  B3DQ32, CARB_BIFLD;  Q8G815, CARB_BIFLO;  
B7GPW2, CARB_BIFLS;  Q8YIC2, CARB_BRUME;  Q8FZJ3, CARB_BRUSU;  
P57244, CARB_BUCAI;  Q8K9Z7, CARB_BUCAP;  P59448, CARB_BUCBP;  
Q8RBK0, CARB_CALS4;  Q9PIL7, CARB_CAMJE;  Q9A4D6, CARB_CAUCR;  
A5CRX5, CARB_CLAM3;  B0REV9, CARB_CLAMS;  Q97FT3, CARB_CLOAB;  
A6LPD9, CARB_CLOB8;  B2UX86, CARB_CLOBA;  B2THH3, CARB_CLOBB;  
C1FNY3, CARB_CLOBJ;  A7GE89, CARB_CLOBL;  B1KT07, CARB_CLOBM;  
Q0TM79, CARB_CLOP1;  Q8XHB3, CARB_CLOPE;  A9KI94, CARB_CLOPH;  
Q0SPY4, CARB_CLOPS;  Q8FT42, CARB_COREF;  P58939, CARB_CORGL;  
C1CXR4, CARB_DEIDV;  Q1IWM0, CARB_DEIGD;  Q9RWK0, CARB_DEIRA;  
B1I4M8, CARB_DESAP;  P63737, CARB_ECO57;  Q8FLB0, CARB_ECOL6;  
P00968, CARB_ECOLI;  Q834E2, CARB_ENTFA;  B1YIR9, CARB_EXIS2;  
C4L5W3, CARB_EXISA;  Q8RG86, CARB_FUSNN;  C1A4I5, CARB_GEMAT;  
O50302, CARB_GEOSE;  Q7VP67, CARB_HAEDU;  Q8RSS3, CARB_HALER;  
B8D1H3, CARB_HALOH;  Q9HP43, CARB_HALSA;  Q9ZKT2, CARB_HELPJ;  
O25577, CARB_HELPY;  A2BJL3, CARB_HYPBU;  A6WCC6, CARB_KINRD;  
B2GI87, CARB_KOCRD;  Q1IPK2, CARB_KORVE;  Q5FJC0, CARB_LACAC;  
Q038Z2, CARB_LACC3;  B3WEF5, CARB_LACCB;  Q047M8, CARB_LACDB;  
B2GD06, CARB_LACF3;  A8YVZ3, CARB_LACH4;  Q74J34, CARB_LACJO;  
Q9CFV2, CARB_LACLA;  O32771, CARB_LACLM;  Q02YG5, CARB_LACLS;  
P77886, CARB_LACPL;  A5VHN6, CARB_LACRD;  B2G564, CARB_LACRJ;  
Q1WVA9, CARB_LACS1;  Q38X24, CARB_LACSS;  Q72NF1, CARB_LEPIC;  
Q8F832, CARB_LEPIN;  B1MZ74, CARB_LEUCK;  Q03WW7, CARB_LEUMM;  
Q92AH3, CARB_LISIN;  C1KWD4, CARB_LISMC;  Q71YI1, CARB_LISMF;  
B8DDR7, CARB_LISMH;  Q8Y665, CARB_LISMO;  A0AJU0, CARB_LISW6;  
B1HQC1, CARB_LYSSC;  B9EB69, CARB_MACCJ;  Q8TNY4, CARB_METAC;  
A7I6J1, CARB_METB6;  P13258, CARB_METBA;  Q469Z7, CARB_METBF;  
Q2FLD9, CARB_METHJ;  A2SQ53, CARB_METLZ;  P58944, CARB_METMA;  
A3CU73, CARB_METMJ;  B8GEA3, CARB_METPE;  A5UKG5, CARB_METS3;  
O27077, CARB_METTH;  A0B8K9, CARB_METTP;  C5CCF1, CARB_MICLC;  
Q7U054, CARB_MYCBO;  A4TBY6, CARB_MYCGI;  Q9CCR2, CARB_MYCLE;  
A3PZ65, CARB_MYCSJ;  A1UFK4, CARB_MYCSK;  Q1B9F9, CARB_MYCSS;  
P9WPK2, CARB_MYCTO;  P9WPK3, CARB_MYCTU;  A1T8H1, CARB_MYCVP;  
Q1D6Y8, CARB_MYXXD;  B2A170, CARB_NATTJ;  Q59599, CARB_NEIGO;  
Q9JW02, CARB_NEIMA;  Q9JXW8, CARB_NEIMB;  Q8YQL2, CARB_NOSS1;  
Q04H29, CARB_OENOB;  B9EXM2, CARB_ORYSJ;  Q9CKV0, CARB_PASMU;  
Q03HB0, CARB_PEDPA;  A5D508, CARB_PELTS;  Q6A914, CARB_PROAC;  
P38100, CARB_PSEAE;  B8H8U5, CARB_PSECP;  A8F453, CARB_PSELT;  
Q88DU6, CARB_PSEPK;  Q87WP4, CARB_PSESM;  Q8ZY48, CARB_PYRAE;  
Q8U085, CARB_PYRFU;  Q8XZ83, CARB_RALSO;  A9WSA8, CARB_RENSM;  
Q98I87, CARB_RHILO;  Q92PZ4, CARB_RHIME;  Q7UJ58, CARB_RHOBA;  
Q1AVY9, CARB_RUBXD;  Q8Z9L7, CARB_SALTI;  P14846, CARB_SALTY;  
O94313, CARB_SCHPO;  P63738, CARB_SHIFL;  A7X1F4, CARB_STAA1;  
A6U122, CARB_STAA2;  Q2FHN5, CARB_STAA3;  Q2FZ72, CARB_STAA8;  
A5IS88, CARB_STAA9;  Q2YXG5, CARB_STAAB;  Q5HGM9, CARB_STAAC;  
A6QGA4, CARB_STAAE;  P63739, CARB_STAAM;  P63740, CARB_STAAN;  
Q6GHN2, CARB_STAAR;  Q6GA10, CARB_STAAS;  A8Z3P0, CARB_STAAT;  
P58940, CARB_STAAW;  B9DPN2, CARB_STACT;  Q5HPY8, CARB_STAEQ;  
Q8CPJ4, CARB_STAES;  Q4L5Q5, CARB_STAHJ;  Q49WY4, CARB_STAS1;  
Q3K150, CARB_STRA1;  Q8E5F5, CARB_STRA3;  Q8DZQ7, CARB_STRA5;  
Q827Q7, CARB_STRAW;  Q9KXR6, CARB_STRCO;  C0M756, CARB_STRE4;  
A8AX83, CARB_STRGC;  B1W463, CARB_STRGG;  Q8DUP3, CARB_STRMU;  
Q9A0C6, CARB_STRP1;  Q04K48, CARB_STRP2;  P0DA14, CARB_STRP3;  
B5E512, CARB_STRP4;  Q5XCR6, CARB_STRP6;  C1C7Q3, CARB_STRP7;  
P58941, CARB_STRP8;  A2RF60, CARB_STRPG;  B1IC65, CARB_STRPI;  
B8ZJT9, CARB_STRPJ;  Q97QE4, CARB_STRPN;  P0DA15, CARB_STRPQ;  
B2IQ67, CARB_STRPS;  B5XKW2, CARB_STRPZ;  Q8CWR0, CARB_STRR6;  
C0MEH1, CARB_STRS7;  A3CNI5, CARB_STRSV;  Q5M0W9, CARB_STRT1;  
Q5M5F6, CARB_STRT2;  Q03LT8, CARB_STRTD;  B9DRV7, CARB_STRU0;  
C1CEM9, CARB_STRZJ;  C1CL09, CARB_STRZP;  C1CR31, CARB_STRZT;  
Q4J8E8, CARB_SULAC;  C3N664, CARB_SULIA;  C4KHM7, CARB_SULIK;  
C3MQE3, CARB_SULIL;  C3MW21, CARB_SULIM;  C3NH23, CARB_SULIN;  
C3NEM0, CARB_SULIY;  Q59969, CARB_SULSO;  Q970U7, CARB_SULTO;  
Q67Q54, CARB_SYMTH;  Q55756, CARB_SYNY3;  Q9HK17, CARB_THEAC;  
Q9WZ27, CARB_THEMA;  B9KB91, CARB_THENN;  A5IJL8, CARB_THEP1;  
B0KBW4, CARB_THEP3;  B0K4D7, CARB_THEPX;  B9KXM5, CARB_THERP;  
B1L8T8, CARB_THESQ;  P96495, CARB_THET2;  Q5SKN1, CARB_THET8;  
Q97AJ3, CARB_THEVO;  P46056, CARB_TRICU;  Q9KPH9, CARB_VIBCH;  
Q87SF3, CARB_VIBPA;  Q8DEM2, CARB_VIBVU;  Q7MNU0, CARB_VIBVY;  
P58942, CARB_XANAC;  P58943, CARB_XANCP;  Q9PEC1, CARB_XYLFA;  
Q87EB8, CARB_XYLFT;  P03965, CARB_YEAST;  Q8ZIL4, CARB_YERPE;  
O50236, CARB_ZYMMO;  Q9K8V6, CARX_BACHD;  P36838, CARX_BACSU;  
P54324, CARX_GEOSE;  O08317, CARX_LACPL;  Q9K8V7, CARY_BACHD;  
P18185, CARY_BACSU;  Q9ZB63, CARY_GEOSE;  Q9RLS9, CARY_LACPL;  
P20054, PYR1_DICDI;  P05990, PYR1_DROME;  O93937, PYR1_EMENI;  
P27708, PYR1_HUMAN;  P08955, PYR1_MESAU;  B2RQC6, PYR1_MOUSE;  
Q09794, PYR1_SCHPO;  Q91437, PYR1_SQUAC;  P07259, PYR1_YEAST;  

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