ENZYME entry: EC 6.3.5.6

Accepted Name
Asparaginyl-tRNA synthase (glutamine-hydrolyzing).
Alternative Name(s)
Asp-AdT.
Asp-tRNA(Asn) amidotransferase.
Aspartyl-tRNA(Asn) amidotransferase.
Reaction catalysed
ATP + aspartyl-tRNA(Asn) + L-glutamine <=> ADP + phosphate + asparaginyl-tRNA(Asn) + L-glutamate
Comment(s)
  • This reaction forms part of a two-reaction system for producing asparaginyl-tRNA in Deinococcus radiodurans and other organisms lacking a specific enzyme for asparagine synthesis.
  • In the first step, a non-discriminating ligase (EC 6.1.1.23) mischarges tRNA(Asn) with aspartate, leading to the formation of aspartyl-tRNA(Asn).
  • The aspartyl-tRNA(Asn) is not used in protein synthesis until the present enzyme converts it into asparaginyl-tRNA(Asn) (aspartyl- tRNA(Asp) is not a substrate for this reaction).
  • Ammonia or asparagine can substitute for the preferred substrate glutamine.
Cross-references
BRENDA6.3.5.6
EC2PDB6.3.5.6
ExplorEnz6.3.5.6
PRIAM enzyme-specific profiles6.3.5.6
KEGG Ligand Database for Enzyme Nomenclature6.3.5.6
IUBMB Enzyme Nomenclature6.3.5.6
IntEnz6.3.5.6
MEDLINEFind literature relating to 6.3.5.6
MetaCyc6.3.5.6

View entry in original ENZYME format
View entry in raw text format (no links)
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.3.5.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.3.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.-.-.-