ID   6.3.5.6
DE   Asparaginyl-tRNA synthase (glutamine-hydrolyzing).
AN   Asp-AdT.
AN   Asp-tRNA(Asn) amidotransferase.
AN   Aspartyl-tRNA(Asn) amidotransferase.
CA   ATP + aspartyl-tRNA(Asn) + L-glutamine + H(2)O = ADP + phosphate +
CA   asparaginyl-tRNA(Asn) + L-glutamate.
CC   -!- This reaction forms part of a two-reaction system for producing
CC       asparaginyl-tRNA in Deinococcus radiodurans and other organisms
CC       lacking a specific enzyme for asparagine synthesis.
CC   -!- In the first step, a non-discriminating ligase (EC 6.1.1.23)
CC       mischarges tRNA(Asn) with aspartate, leading to the formation of
CC       aspartyl-tRNA(Asn).
CC   -!- The aspartyl-tRNA(Asn) is not used in protein synthesis until the
CC       present enzyme converts it into asparaginyl-tRNA(Asn) (aspartyl-
CC       tRNA(Asp) is not a substrate for this reaction).
CC   -!- Ammonia or asparagine can substitute for the preferred substrate
CC       glutamine.
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