ENZYME entry: EC 6.5.1.3

Accepted Name
RNA ligase (ATP).
Alternative Name(s)
Polyribonucleotide synthase (ATP).
Ribonucleic ligase.
Reaction catalysed
ATP + (ribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(ribonucleotide)(m) <=> (ribonucleotide)(n+m) + AMP + diphosphate
Comment(s)
  • The enzyme catalyzes the ligation of RNA strands with 3'-hydroxyl and 5'-phosphate termini, forming a phosphodiester and sealing certain types of single-strand breaks in RNA.
  • Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a lysine residue.
  • The adenylate group is then transferred to the 5'-phosphate terminus of the substrate, forming the capped structure 5'-(5'-diphosphoadenosine)-(RNA).
  • Finally, the enzyme catalyzes a nucleophilic attack of the 3'-OH terminus on the capped terminus, which results in formation of the phosphodiester bond and release of the adenylate.
Cross-references
BRENDA6.5.1.3
EC2PDB6.5.1.3
ExplorEnz6.5.1.3
PRIAM enzyme-specific profiles6.5.1.3
KEGG Ligand Database for Enzyme Nomenclature6.5.1.3
IUBMB Enzyme Nomenclature6.5.1.3
IntEnz6.5.1.3
MEDLINEFind literature relating to 6.5.1.3
MetaCyc6.5.1.3
UniProtKB/Swiss-Prot
P86927, RLGM1_TRYB2;  P86926, RLGM1_TRYBB;  P86925, RLGM2_TRYB2;  
P86924, RLGM2_TRYBB;  P32277, RLIG2_BPT4;  P00971, RLIG_BPT4;  
P41476, RNL1_NPVAC;  B0WCT9, RTCB1_CULQU;  B0EAV2, RTCB1_ENTDS;  
C4M244, RTCB1_ENTHI;  B0XKF3, RTCB2_CULQU;  B0EIW5, RTCB2_ENTDS;  
C4M6T2, RTCB2_ENTHI;  Q17FP1, RTCB_AEDAE;  Q7Q412, RTCB_ANOGA;  
Q5E9T9, RTCB_BOVIN;  C3YN79, RTCB_BRAFL;  A8QC60, RTCB_BRUMA;  
P90838, RTCB_CAEEL;  A8JC00, RTCB_CHLRE;  Q6NZS4, RTCB_DANRE;  
Q54Y09, RTCB_DICDI;  Q9VIW7, RTCB_DROME;  Q9Y3I0, RTCB_HUMAN;  
Q4R6X4, RTCB_MACFA;  C1E9Y5, RTCB_MICCC;  C1MI97, RTCB_MICPC;  
A9UXG6, RTCB_MONBE;  Q99LF4, RTCB_MOUSE;  A7RKF6, RTCB_NEMVE;  
A4S3S3, RTCB_OSTLU;  Q00ZY2, RTCB_OSTTA;  A9CB42, RTCB_PAPAN;  
Q19PY3, RTCB_PIG;  Q4YUZ9, RTCB_PLABA;  Q8IIU6, RTCB_PLAF7;  
B3L4K9, RTCB_PLAKH;  Q7RI54, RTCB_PLAYO;  Q6AYT3, RTCB_RAT;  
B8LBM8, RTCB_THAPS;  Q4U923, RTCB_THEAN;  Q4N1R8, RTCB_THEPA;  
B3RID0, RTCB_TRIAD;  Q561P3, RTCB_XENTR;  P43075, TRNL_CANAL;  
Q10313, TRNL_SCHPO;  P09880, TRNL_YEAST;  

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.5.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.5.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.-.-.-