ID   6.5.1.7
DE   DNA ligase (ATP, ADP or GTP).
CA   (1) ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CA   (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.
CA   (2) ADP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CA   (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + phosphate.
CA   (3) GTP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CA   (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + GMP + diphosphate.
CC   -!- The enzymes from the archaea Hyperthermus butylicus and
CC       Sulfophobococcus zilligii are active with ATP, ADP or GTP.
CC   -!- They show no activity with NAD(+).
CC   -!- The enzyme catalyzes the ligation of DNA strands with 3'-hydroxyl and
CC       5'-phosphate termini, forming a phosphodiester and sealing certain
CC       types of single-strand breaks in duplex DNA.
CC   -!- Catalysis occurs by a three-step mechanism, starting with the
CC       activation of the enzyme by ATP, ADP, or GTP, forming a phosphoramide
CC       bond between adenylate/guanylate and a lysine residue.
CC   -!- The nucleotide is then transferred to the 5'-phosphate terminus of
CC       the substrate, forming the capped structure
CC       5'-(5'-diphosphoadenosine/guanosine)-[DNA].
CC   -!- Finally, the enzyme catalyzes a nucleophilic attack of the 3'-OH
CC       terminus on the capped terminus, which results in formation of the
CC       phosphodiester bond and release of the nucleotide.
CC   -!- Different from EC 6.5.1.1 and EC 6.5.1.6, which cannot utilize GTP.
DR   Q9YD18, DNLI_AERPE ;  A2BJX6, DNLI_HYPBU ;  A3DP49, DNLI_STAMF ;
DR   D2CJS7, DNLI_SULZI ;
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