ENZYME

ENZYME entry: EC 6.6.1.2

Accepted Name

Cobaltochelatase.

Alternative Name(s)

Hydrogenobyrinic acid a,c-diamide cobaltochelatase.

Reaction catalysed

ATP + hydrogenobyrinic acid a,c-diamide + Co(2+) + H(2)O <=> ADP + phosphate + cob(II)yrinic acid a,c-diamide + H(2)

Comment(s)

This enzyme, which forms part of the aerobic cobalamin biosynthesis pathway, is a type I chelatase, being heterotrimeric and ATP- dependent.
It comprises two components, one of which corresponds to CobN and the other is composed of two polypeptides, specified by cobS and cobT in Pseudomonas denitrificans, and named CobST.
Hydrogenobyrinic acid is a very poor substrate.
ATP can be replaced by dATP or CTP but the reaction proceeds more slowly.
CobN exhibits a high affinity for hydrogenobyrinic acid a,c-diamide.
The oligomeric protein CobST possesses at least one sulfhydryl group that is essential for ATP-binding.
Once the Co(2+) is inserted, the next step in the pathway ensures that the cobalt is ligated securely by reducing Co(II) to Co(I); this step is carried out by EC 1.16.8.1.

Cross-references

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature