ID   1.1.98.6
DE   ribonucleoside-triphosphate reductase (formate).
AN   anaerobic ribonucleoside-triphosphate reductase.
CA   a ribonucleoside 5'-triphosphate + formate + H(+) =
CA   a 2'-deoxyribonucleoside 5'-triphosphate + CO2 + H2O.
CC   -!- The enzyme, which is expressed in the bacterium Escherichia coli
CC       during anaerobic growth, contains an iron sulfur center.
CC   -!- The active form of the enzyme contains an oxygen-sensitive glycyl
CC       (1-amino-2-oxoethan-1-yl) radical that is generated by the activating
CC       enzyme NrdG via chemistry involving S-adenosylmethionine (SAM) and a
CC       [4Fe-4S] cluster.
CC   -!- The glycyl radical is involved in generation of a transient thiyl
CC       (sulfanyl) radical on a cysteine residue, which attacks the
CC       substrate, forming a ribonucleotide 3'-radical, followed by water
CC       loss to form a ketyl (alpha-oxoalkyl) radical.
CC   -!- The ketyl radical gains an electron from a cysteine residue and a
CC       proton from formic acid, forming 3'-keto-deoxyribonucleotide and
CC       generating a thiosulfuranyl (1lambda(4)-disulfan-1-yl) radical bridge
CC       between methionine and cysteine residues.
CC   -!- Oxidation of formate by the thiosulfuranyl radical results in the
CC       release of CO2 and regeneration of the thiyl radical. cf. EC 1.17.4.1
CC       and EC 1.17.4.2.
DR   P07071, NRDD_BPT4  ;  P28903, NRDD_ECOLI ;  P43752, NRDD_HAEIN ;
DR   Q9L646, NRDD_SALTY ;
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