ID 1.1.99.28 DE glucose-fructose oxidoreductase. CA D-fructose + D-glucose = D-glucono-1,5-lactone + D-sorbitol. CC -!- D-mannose, D-xylose, D-galactose, 2-deoxy-D-glucose and L-arabinose CC will function as aldose substrates, but with low affinities. CC -!- The ketose substrate must be in the open-chain form. CC -!- The apparent affinity for fructose is low, because little of the CC fructose substrate is in the open-chain form. CC -!- Xylulose and glycerone (dihydroxyacetone) will replace fructose, CC but they are poor substrates. CC -!- The enzyme from Zymomonas mobilis contains tightly bound NADP(+). DR Q07982, GFO_ZYMMO ; //