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ENZYME entry: EC

Accepted Name
NADH-dependent peroxiredoxin.
Alternative Name(s)
alkyl hydroperoxide reductase.
Reaction catalysed
a hydroperoxide + H(+) + NADH <=> an alcohol + H2O + NAD(+)
  • Peroxiredoxins (Prxs) are a ubiquitous family of antioxidant proteins.
  • They can be divided into three classes: typical 2-Cys, atypical 2-Cys and 1-Cys peroxiredoxins.
  • The peroxidase reaction comprises two steps centered around a redox- active cysteine called the peroxidatic cysteine.
  • All three peroxiredoxin classes have the first step in common, in which the peroxidatic cysteine attacks the peroxide substrate and is oxidized to S-hydroxycysteine (a sulfenic acid).
  • The second step of the peroxidase reaction, the regeneration of cysteine from S-hydroxycysteine, distinguishes the three peroxiredoxin classes.
  • For typical 2-Cys Prxs, in the second step, the peroxidatic S-hydroxycysteine from one subunit is attacked by the 'resolving' cysteine located in the C-terminus of the second subunit, to form an intersubunit disulfide bond, which is then reduced by one of several cell-specific thiol-containing reductants completing the catalytic cycle.
  • In the atypical 2-Cys Prxs, both the peroxidatic cysteine and its resolving cysteine are in the same polypeptide, so their reaction forms an intrachain disulfide bond.
  • The 1-Cys Prxs conserve only the peroxidatic cysteine, so its regeneration involves direct interaction with a reductant molecule.
  • This bacterial peroxiredoxin differs from most other forms by comprising two types of subunits.
  • One subunit (AhpC) is a typical 2-Cys peroxiredoxin.
  • Following the reduction of the substrate, one AhpC subunit forms a disulfide bond with an identical unit.
  • The disulfide bond is reduced by the second type of subunit (AhpF).
  • This second subunit is a flavin-containing protein that uses electrons from NADH to reduce the cysteine residues on the AhpC subunits back to their active state.
  • Formerly EC
PRIAM enzyme-specific profiles1.11.1.26
KEGG Ligand Database for Enzyme Nomenclature1.11.1.26
IUBMB Enzyme Nomenclature1.11.1.26
MEDLINEFind literature relating to
Rhea expert-curated reactions1.11.1.26
P83117, AHPC_DELAC;  P0AE10, AHPC_ECO57;  P0AE09, AHPC_ECOL6;  

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