|Cleavage of cis-1,4-polyisoprene polymers into a mixture of compounds, including a C(20) compound ((4Z,8Z,12Z,16Z,20Z,24Z)-4,8,12,16,20,24-hexamethyl-28-oxononacosa-4,8,12,16,20,24-hexaenal), a C(25) compound ((4Z,8Z,12Z,16Z,20Z)-4,8,12,16,20-pentamethyl-24-oxopentacosa-4,8,12,16,20-pentaenal), a C(30) compound ((4Z,8Z,12Z,16Z)-4,8,12,16-tetramethyl-20-oxohenicosa-4,8,12,16-tetraenal), and higher molecular weight compounds|
- The enzyme catalyzes the cleavage of the double bonds in natural and
synthetic rubber, producing a mixture of C(20), C(25), C(30),
and higher oligo-isoprenoids with ketone and aldehyde groups at their
- Two unrelated bacterial enzymes are known to possess this activity -
the enzyme from Streptomyces sp. K30 (Lcp) contains a b-type
cytochrome, while the enzyme from Xanthomonas sp. 35Y, (RoxB)
contains two c-type cytochromes.
- Both enzymes attack the substrate at random locations, and are not
able to cleave the C(35) or smaller products into shorter fragments.